Lipids

, Volume 25, Issue 7, pp 401–405 | Cite as

Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry

  • William K. Rohwedder
  • Sandra M. Duval
  • Darhal J. Wolf
  • Edward A. Emken
Methods

Abstract

An analytical method that was developed to analyze deuterium-labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new computer and a hard-wave adder have been utilized to assure reliable data acquisition. Relative standard derivations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation.

Abbreviations

ADC

analog-to-digital converte

CI

chemical ionization

DAC

digital-to-analog converter

GC

gas chromatography

MID

multiple-ion-monitoring

RSD

relative standard deviation

TI

total ionization

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Copyright information

© American Oil Chemists’ Society 1990

Authors and Affiliations

  • William K. Rohwedder
    • 1
  • Sandra M. Duval
    • 1
  • Darhal J. Wolf
    • 1
  • Edward A. Emken
    • 1
  1. 1.Vegetable Oil Research, Northern Regional Research Center, Agricultural Research ServiceU.S. Department of AgriculturePeoria

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