Lipids

, Volume 26, Issue 5, pp 395–399

Effect of chitosan feeding on intestinal bile acid metabolism in rats

  • Yasuhiko Fukada
  • Koji Kimura
  • Yoshikazu Ayaki
Article

Abstract

The effect of chitosan feeding (for 21 days) on intestinal bile acids was studied in male rats. Serum cholesterol levels in rats fed a commercial diet low in cholesterol were decreased by chitosan supplementation. Chitosan inhibited the transformation of cholesterol to coprostanol without causing a qualitative change in fecal excretion of these neutral sterols. Increased fiber consumption did not increase fecal excretion of bile acids, but caused a marked change in fecal bile acid composition. Litcholic acid increased sigificantly, deoxycholic acid increased to a leasser extent, whereas hyodeoxycholic acid and the 6β-isomer and 5-epimeric 3α-hydroxy-6-keto-cholanoic acid(s) decreased. The pH in the cecum and colon became elevated by chitosan feeding which affected the conversion of primary bile acids to secondary bile acids in the large intestine. In the cecum, chitosan feeding increased the concentration of α-,β-, and ω-muricholic acids, and lithocholic acid. However, the levels of hyodeoxycholic acid and its 6β-isomer, of monohydroxy-monoketo-cholanoic acids, and of 3α, 6ξ, 7ξ-trihydroxy-cholanoic acid decreased. The data suggest that chitosan feeding affects the metabolism of intestinal bile acids in rats.

Abbreviations

GC/MS

gas-liquid chromatography/mass spectrometry

MC

muricholic acid

HD

hyodeoxydiolic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sugano, M., Fujikawa, T., Hiratsuji, Y., and Hasegawa, Y. (1978)Nutr. Rept. Int. 18, 531–537.Google Scholar
  2. 2.
    Nagyvary, J.J., Falk, J.D., Hill, M.L., Schmidt, M.L., Wilkins, A.K., and Bradbury, E.L. (1979)Nutr. Rept. Int. 20, 677–684.Google Scholar
  3. 3.
    Kobayashi, T., Otsuka, S., and Yugari, Y. (1979)Nutr. Rept. Int. 19, 327–334.Google Scholar
  4. 4.
    Sugano, M., Fujikawa, T., Hiratzuji, Y., Nakashima, K., Fukuda, N., and Hasegawa, Y. (1980)Amer. J. Clin. Nutr. 33, 787–793.PubMedGoogle Scholar
  5. 5.
    Sugano, M., Watanabe, S., Kishi, A., Izume, M., and Ohtakara, A. (1988)Lipids 23, 187–191.PubMedCrossRefGoogle Scholar
  6. 6.
    Vahouny, G.V., Satchithanandam, S., Cassidy, M.M., Lightfoot, F.B., and Furda, I. (1983)Amer. J. Clin. Nutr. 38, 278–284.PubMedGoogle Scholar
  7. 7.
    Samuelsson, B. (1960)J. Biol. Chem. 235, 361–366.Google Scholar
  8. 8.
    Norman, A., and Sjövall, J. (1960)Acta Chem. Scand. 14, 1815–1818.Google Scholar
  9. 9.
    Norman, A., and Sjövall, J. (1958)J. Biol. Chem. 233, 872–885.PubMedGoogle Scholar
  10. 10.
    Hill, M.J. (1986) inDietary Fiber. Basic and Clinical Aspects. Vahouny, G.V., and Kritchevsky, D., eds.) pp. 497–513, Plenum Press, New York.Google Scholar
  11. 11.
    Palmer, R.H., and Hruban, Z. (1966)J. Clin. Invest. 45, 1255–1267.PubMedGoogle Scholar
  12. 12.
    Heaton, K.W. (1986) inDietary Fiber. Basic and Clinical Aspects. (Vahouny, G.V., and Kritchevsky, D., eds.) pp. 411–426, Plenum Press, New York.Google Scholar
  13. 13.
    Story, J.A., and Thomas, J.N. (1982) inDietary Fiber in Health and Disease (Vahouny, G.V., and Kritchevsky, D., eds.) pp. 193–201, Plenum, New York.Google Scholar
  14. 14.
    Hylemon, P.B. (1985) inSterols and Bile Acids (Danielsson, H., and Sjövall, J., eds.) pp. 331–343, Elsevier Sciece Publishers, Amsterdam.Google Scholar
  15. 15.
    Vahouny, G.V., Khalafi, R., Satchithanandam, S., Watkins, D.W., Story, J.A., Cassidy, M.M., and Kritchevsky, D. (1987)J. Nutr. 117, 2009–2015.PubMedGoogle Scholar
  16. 16.
    Thomas, P.J., Hsia, S.L., Matschiner, J.T., Thayer, S.A., Elliott, W.H., Doisy, Jr., E.A., and Doisy, E.A. (1965)J. Biol. Chem. 240, 1059–1063.PubMedGoogle Scholar
  17. 17.
    Bergström, S., Goransson, A., and Samuelsson, B. (1959)Acta Chem. Scand. 13, 1761–1766.CrossRefGoogle Scholar
  18. 18.
    Dietschy, J.M., and Siperstein, M.D. (1965)J. Clin. Invest. 44, 1311–1327.PubMedGoogle Scholar
  19. 19.
    Ayaki, Y., Endo, S., Ogura, Y., and Ogura, M. (1988)Lipids 23, 169–177.PubMedCrossRefGoogle Scholar
  20. 20.
    Ayaki, Y., Tsuma-Date, T., Endo, S., and Ogura, M. (1981)Steroids 38, 495–509.PubMedCrossRefGoogle Scholar
  21. 21.
    Haslewood, G.A.D. (1967) inBile Salts (Haslewood, G.A.D., ed.) pp. 6–16, Methuen & Co. Ltd., London.Google Scholar
  22. 22.
    Myant, N.B. (1981) inThe Biology of Cholesterol and Related Steroids (Myant, N.B., ed.) pp. 125–159; 449–504, William Heinenmann Medical Books Ltd., London.Google Scholar
  23. 23.
    Swell, L., Trout, Jr. E.C., Hopper, J.R., Field, Jr. H., and Treadwell, C.R. (1958)J. Biol. Chem. 232, 108.Google Scholar
  24. 24.
    Portman, O.W. (1958)Arch. Biochem. Biophys. 78, 125–137.PubMedCrossRefGoogle Scholar
  25. 25.
    Bergström, S., Rottenberg, M., and Sjövall, J. (1953)Hoppe-Seyler's Z. Physiol. Chem. 295, 278–285.PubMedGoogle Scholar
  26. 26.
    Mahowald, T.A., Matschiner, J.T., Hsia, S.L., Richter, R., Doisy, Jr., E.A., Elliott, W.H., and Doisy, E.A. (1957)J. Biol. Chem. 225, 781–793.PubMedGoogle Scholar
  27. 27.
    Elliott, W.H. (1985) inSterols and Bile Acids (Danielsson, H., and Sjövall, J., eds.) pp. 303–329, Elsevier Science Publishers, Amsterdam.Google Scholar
  28. 28.
    Zimniak, P., Holsztynska, E.J., Lester, R., Waxman, D.J., and Radominska, A. (1989)J. Lipid Res. 30, 907–918.PubMedGoogle Scholar
  29. 29.
    Macdonald, I.A., Singh, G., Mahony, D.E., and Meier, C.E. (1978)Steroids 32, 245–256.PubMedCrossRefGoogle Scholar
  30. 30.
    Cohen, B.I., Raicht, R.F., Salen, G., and Mosbach, E.H. (1975)Anal. Biochem. 64, 565–577.CrossRefGoogle Scholar
  31. 31.
    Sacquet, E., Van Heijenoort, Y., Riottot, M., and Leprince, C. (1975)Biochim. Biophys. Acta 380, 52–65.PubMedGoogle Scholar
  32. 32.
    Madsen, D., Beaver, M., Chang, L., Bruckner-Kardoss, E., and Wostmann, B. (1976)J. Lipids Res. 17, 107–111.Google Scholar
  33. 33.
    Brydon, W.G., Tadesse, K., Eastwood, M.F., and Lawson, M.E., (1980)Br. J. Nutr. 43, 101–106.PubMedCrossRefGoogle Scholar
  34. 34.
    Sacquet, E.C., Raibaud, P.M., Mejean, C., Riottot, M.J., Leprince, C., and Leglise, P.C. (1979)Appl. Environ. Microbiol. 37, 1127–1131.PubMedGoogle Scholar
  35. 35.
    Eyssen, H., De Pauw, G., Stragier, J., and Verhulst, A. (1983)Appl. Environ. Microbiol. 45, 141–147.PubMedGoogle Scholar
  36. 36.
    Story, J.A. (1986) inDietary Fiber. Basic and Clinical Aspects. (Vahouny, G.V., and Kritchevsky, D., eds.) pp. 253–264, Plenum Press, New York.Google Scholar

Copyright information

© American Oil Chemists’ Society 1991

Authors and Affiliations

  • Yasuhiko Fukada
    • 1
  • Koji Kimura
    • 1
  • Yoshikazu Ayaki
    • 1
  1. 1.Department of BiochemistryTottori University School of MedicineTottoriJapan

Personalised recommendations