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Lipids

, Volume 28, Issue 11, pp 987–993 | Cite as

Preferential reduction in adipose tissue α-linolenic acid (18∶3ω3) during very low calorie dieting despite supplementation with 18∶3ω3

  • Anna B. Tang
  • Karen Y. Nishimura
  • Stephen D. Phinney
Article

Abstract

We have previously reported that the relative content of 18∶3ω3 in adipose triglyceride (TG) of women was reduced following major weight loss while on a very low calorie diet (VLCD). In an attempt to prevent this loss of 18∶3ω3 reserves, we have tested two VLCD supplemented with varying amounts of 18∶3ω3. The formula (FORM) and food VLCD (2.1–3.0 MJ or 500–700 kcal/d) contained 20 g/d of fat and provided the recommended dietary allowance for minerals and vitamins. FORM subjects (Group 1) were 5 women [initial body mass index (BMI) of 36.8, 168% ideal body weight (IBW) who received 20 g/d of canola oil (1.6 g 18∶3ω3). Their mean weight loss was 23.9 kg in a 4–5 mon period. Food VLCD subjects (Group 2) were 6 women (BMI 33.9, 155% IBW) supplemented with 2 g/d of linseed oil (1.1 g 18∶3ω3). Their mean weight loss was 17.4 kg in a 2–3 mon period. Needle biopsies of adipose tissue were obtained from Group 1 before, at midpoint and after weight loss; and from Group 2 before and after weight loss. The adipose TG and serum (Group 1) were separated and their fatty acid composition determined by thin-layer and gas chromatography. In Group 1, adipose 18∶3ω3 fell from 0.65 to 0.59 wt%, then to 0.52 wt% during weight loss. In Group 2, it fell from 0.77 to 0.64 wt%. The fall in adipose 18∶3ω3 with weight loss was significant atP=0.01 (Group 1) andP<0.01 (Group 2). There were no differences between responses to the 1.1 g/d or 1.6 g/d 18∶3ω3 supplements. The relative content of 18∶3ω3 in serum free fatty acids from Group 1 was reduced after major weight loss. Thus, in both groups the ω3 supplementation did not help to maintain adipose tissue 18∶3ω3 during rapid weight loss, and its decrement may affect circulating lipid pools. As adipose 18∶2ω6 did not change with weight loss, this reduction in the ratio of ω6 precursor to ω3 precursor could eventually alter the balance of their products as well.

Keywords

Ideal Body Weight Rapid Weight Loss Serum Free Fatty Acid Initial Body Mass Index Major Weight Loss 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BMI

body mass index (weight in kilogram divided by the square of height in meters)

DHA

docosahexaenoic acid (22∶6ω3)

EPA

eicosapentaenoic acid (20∶5ω3)

EFA

essential fatty acid

FFM

fat-free mass

GC

gas chromatography

IBW

ideal body weight

PL

phospholipids

RDA

recommended dietary allowance

TG

triglyceride

TLC

thin-layer chromatography

VLCD

very low calorie diet(s)

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Copyright information

© American Oil Chemists’ Society 1993

Authors and Affiliations

  • Anna B. Tang
    • 1
  • Karen Y. Nishimura
    • 2
  • Stephen D. Phinney
    • 1
  1. 1.Division of Clinical Nutrition and Metabolism, Department of MedicineUniversity of California at DavisDavis
  2. 2.School of MedicineUniversity of California at DavisDavis

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