Lipids

, Volume 28, Issue 12, pp 1069–1074 | Cite as

Effects oftrans fatty acids on lipid accumulation in 3T3-L1 cells

  • Kalpana Panigrahi
  • Joseph Sampugna
Article

Abstract

Previous work had shown that dietarytrans fatty acids (tFA) resulted in decreased fat deposition in adipose tissue. This study was conducted to see iftFA influence lipid accumulation in Swiss mouse fibroblast 3T3-L1 cells, which are widely used as an adipocyte model. Cells were cultured in the presence of experimental or control growth media supplemented with fatty acids complexed to bovine serum albumin. Fatty acid compositions of experimental and control growth media were similar except that the octadecenoates in the control growth media werecis fatty acids, whereas those in the experimental media contained bothcis andtrans fatty acids. Cell-conditioned media and cellular lipids at the preadipocyte and differentiating adipocyte stages were analyzed. At both stages of development, less fat accumulated, in cells cultured in the presence oftFA, due primarily to a decrease in the nonpolar lipid content of cells exposed totFA, and linoleate to arachidonate ratios were higher in cells supplemented withtFA. Calculations comparing sums of saturated and monounsaturated fatty acids in cells at the differentiating adipocyte stage suggested thattFA may have replaced monoun-saturated fatty acids in the nonpolar lipid fraction and saturated fatty acids in the polar lipid fraction. The results of these studies are in good agreement with thein vivo effects oftFA seen in previous work with mouse adipose tissue. It was concluded that the 3T3-L1in vitro model is an appropriate system for further studies oftFA and lipid metabolism in adipose tissue.

Abbreviations

BSA

bovine serum albumin

cAMP

3′, 5′-cyclic adenosine monophosphate

CGM

control growth medium

DA

differentiating adipocytes

DEX

dexamethasone

DMEM

Dulbecco's modified Eagle medium

EGM

experimental growth medium

FAME

fatty acid methyl esters

FCS

fetal calf serum

GM

growth medium

HDL

high density lipoprotein

LDL

low density lipoprotein

LPSR-1

low protein serum replacement

MIX

3-isobutyl-1-methyl xanthine

NEAA

nonessential amino acids

PA

preadipocytes

PBS

phosphate buffered saline

tFA

trans fatty acids

TLC

thin-layer chromatography

3T3-L1

subclone of Swiss mouse embryo 3T3 fibroblasts

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Copyright information

© American Oil Chemists’ Society 1993

Authors and Affiliations

  • Kalpana Panigrahi
    • 1
  • Joseph Sampugna
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of MarylandCollege Park

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