Lipids

, Volume 30, Issue 1, pp 15–21 | Cite as

Trans fatty acid isomers in Canadian human milk

  • Z. -Y. Chen
  • G. Pelletier
  • R. Hollywood
  • W. M. N. Ratnayake
Article

Abstract

The fatty acid composition, totaltrans content (i.e., sum of all the fatty acids which may have one or moretrans double bonds) and geometric and positional isomer distribution of unsaturated fatty acids of 198 human milk samples collected in 1992 from nine provinces of Canada were determined using a combination of capillary gas-liquid chromatography and silver nitrate thin-layer chromatography. The mean totaltrans fatty acid content was 7.19±3.03% of the total milk fatty acids and ranged from 0.10 to 17.15%. Twenty-five of the 198 samples contained more than 10% totaltrans fatty acids, and thirteen samples contained less than 4%. Totaltrans isomers of linoleic acid were 0.89% of the total milk fatty acids with 18∶2Δ9c, 13t being the most prevalent isomer, followed by 18∶2Δ9c, 12t and 18∶2Δ9t, 12c. Using the totaltrans values in human milk determined in the present study, the intake of totaltrans fatty acids from various dietary sources by Canadian lactating women was estimated to be 10.6±3.7 g/person/d, and in some individuals, the intake could be as high as 20.3 g/d. The 18∶1trans isomer distribution differed from that of cow's milk fat but was remarkably similar to that in partially hydrogenated soybean and canola oils, suggesting that partially hydrogenated vegetable oils are the major source of thesetrans fatty acids.

Abbreviations

AgNO3-TLC

silver nitrate thin-layer chromatography

c

cis

FAME

fatty acid methyl esters

GLC

gas-liquid chromatography

HTM

high-trans milk

LTM

low-trans milk

MTM

medium-trans milk

PHCO

partially hydrogenated canola oil

PHSO

partially hydrogenated soybean oil

t

trans

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References

  1. 1.
    Mensink, R.P., and Katan, M.B. (1990)N. Engl. J. Med. 323, 439–445.PubMedCrossRefGoogle Scholar
  2. 2.
    Judd, J.T., Clevidence, B.A., Muesing, R.A., Wittes, J., Sunkin, M.E., and Podczasy, J.J. (1994)Am. J. Clin. Nutr. 59, 861–868.PubMedGoogle Scholar
  3. 3.
    Willet, W.C., Stampfer, M.J., Manson, J.E., Golditz, G.A., Speizer, F.E., Rosner, B.A., Sampson, L.A., and Hennekens, C.H. (1993)Lancet 341, 581–585.CrossRefGoogle Scholar
  4. 4.
    Siguel, E.N., and Lerman, R.H. (1993)Am. J. Cardiol. 71, 916–920.PubMedCrossRefGoogle Scholar
  5. 5.
    Emken, E.A. (1983)J. Am. Oil. Chem. Soc. 60, 995–1004.Google Scholar
  6. 6.
    Senti, F.,Health Effects of Dietary transFatty Acids, Bethesda, MD, Federation of American Societies for Experimental Biology, August 1985 (Report of HHS Contract No. FDA 223-87-2020).Google Scholar
  7. 7.
    Koletzko, B. (1992)Acta. Paediatr. 81, 302–306.PubMedGoogle Scholar
  8. 8.
    Koletzko, B., and Muller, J. (1990)Biol. Neonate 57, 172–178.PubMedCrossRefGoogle Scholar
  9. 9.
    Anderson, R.L., Fullmer, C.S., and Hollenbach, E.J. (1975)J. Nutr. 105, 393–400.PubMedGoogle Scholar
  10. 10.
    Beyers, E.C., and Emken, E.A. (1991)Biochim. Biophys. Acta 1082, 275–284.PubMedGoogle Scholar
  11. 11.
    Holman, R.T., Pusch, F., Svingen, B., and Dutton, H.J. (1991)Proc. Natl. Acad. Sci. (USA) 88, 4830–4834.CrossRefGoogle Scholar
  12. 12.
    Hill, E.G., Johnson, S.B., Lawson, L.D., Mahfouz, M.M., and Holman, R.T. (1982)Proc. Natl. Acad. Sci. (USA) 79, 953–957.CrossRefGoogle Scholar
  13. 13.
    Ratnayake, W.M.N., Hollywood, R., O'Grady, E., and Pelletier, G. (1993)J. Am. Coll. Nutr. 12, 651–660.PubMedGoogle Scholar
  14. 14.
    Ratnayake, W.M.N., and Pelletier, G. (1992)J. Am. Oil. Chem. Soc. 69, 95–105.Google Scholar
  15. 15.
    Ratnayake, W.M.N., Chen, Z.Y., Pelletier, G., and Weber, D. (1994)Lipids 29, 707–714.PubMedGoogle Scholar
  16. 16.
    Picciano, M.F., and Perkins, E.G. (1977)Lipids 12, 407–408.PubMedCrossRefGoogle Scholar
  17. 17.
    Koletzko, B., Maria, M., and Bremer, H.J. (1988)Am. J. Clin. Nutr. 47, 954–959.PubMedGoogle Scholar
  18. 18.
    Atichison, J.M., Kunkley, W.L., Canotly, N.L., and Smith, L.M. (1977)Am. J. Clin. Nutr. 30, 2006–2015.Google Scholar
  19. 19.
    Chappell, J.E., Clandinin, M.T., and Kearney-Volpe, K. (1985)Am. J. Clin. Nutr. 42, 49–56.PubMedGoogle Scholar
  20. 20.
    Ohlrogge, J.B., Emken, E.A., and Gulley, R.M. (1981)J. Lipid Res. 22, 955–960.PubMedGoogle Scholar
  21. 21.
    Teter, B.B., Sampugna, J., and Keeney, M. (1990)J. Nutr. 120, 818–824.PubMedGoogle Scholar
  22. 22.
    Teter, B.B., Sampugna, J., and Keeney, M. (1992)Lipids 27, 912–916.PubMedGoogle Scholar
  23. 23.
    Pettersen, J., and Opstvedt, J. (1991)Lipids 26, 711–717.PubMedGoogle Scholar
  24. 24.
    Mes, J. (1981)Internat. J. Environ. Anal. Chem. 9, 283–299.Google Scholar
  25. 25.
    Chen, Z.Y., and Cunnane, S.C. (1992)Am. J. Physiol. 27, 912–916.Google Scholar
  26. 26.
    Ratnayake, W.M.N., and Beare-Rogers, J.L. (1990)J. Chromatogr. Sci. 28, 633–639.Google Scholar
  27. 27.
    Ackman, R.G., Sebedio, J.L., and Ratnayake, W.N. (1981)Methods Enzymol. 72, 253–276.PubMedCrossRefGoogle Scholar
  28. 28.
    Craig-Schmidt, M.C., Weete, J.D., Faircloth, S.A., Wickwire, M.A., and Livant, E.J. (1984)Am. J. Clin. Nutr. 39, 778–786.PubMedGoogle Scholar
  29. 29.
    Health and Welfare Canada (1990)Nutrition Recommendations: The Report of the Scientific Review Committee, Canadian Government Publishing Centre, Ottawa, Canada.Google Scholar
  30. 30.
    Brisson, G.J. (1981) inLipids in Human Nutrition, Jack K Burgess, Inc. pp. 41–71, Englewood.Google Scholar
  31. 31.
    Finley, D.A., Lonnerdal, B., Dewey, K.G., and Grivetti, L.E. (1985)Am. J. Clin. Nutr. 41, 787–800.PubMedGoogle Scholar
  32. 32.
    Hunderieser, K.E., Clark, R.M., and Brown, P.B. (1983)J. Ped. Gastro. Nutr. 2, 635–639.CrossRefGoogle Scholar
  33. 33.
    Gregory, J., Foster, K., Tyler, H., and Wiseman, M. (1990)The Dietary and Nutritional Survey of British Adults, The Social Survey Division, Office of Population Censes and Survey, The United Kingdom.Google Scholar
  34. 34.
    Enig, M.G., Atal, S., Keeney, L., and Sampugna, J. (1990)J. Am. Coll. Nutr. 9, 471–486.PubMedGoogle Scholar
  35. 35.
    Hunter, J.E., and Applewhite, T.H. (1986)Am. J. Clin. Nutr. 44, 707–717.PubMedGoogle Scholar
  36. 36.
    Hunter, J.E., and Applewhite, T.H. (1991)Am. J. Clin. Nutr. 54, 363–369.PubMedGoogle Scholar
  37. 37.
    Jensen, R.G., Hagerty, M.M., and McMahon, K.E. (1978)Am. J. Clin. Nutr. 31, 990–1016.PubMedGoogle Scholar
  38. 38.
    Insull, W., Hirsh, T.J., James, T., and Ahrens, E.H. (1959)J. Clin. Invest. 28, 443–450.Google Scholar
  39. 39.
    Martin, F.C., Bougnoux, P., Fignon, A., Theret, V., Antoine, J.M., Lamisse, F., and Gouet, C. (1993)Am. J. Clin. Nutr. 58, 653–659.PubMedGoogle Scholar
  40. 40.
    Dayton, S., Hashimoto, S., Dixon, W., and Pearce, M.L. (1966)J. Lipid Res. 7, 103–106.PubMedGoogle Scholar
  41. 41.
    Beynen, A.C., Herms, R.J.J., and Hautvost, J.G.A.J. (1980)Am. J. Clin. Nutr. 33, 81–85.PubMedGoogle Scholar
  42. 42.
    Hirsh, J.J., Farquhar, W., Ahrens, E.H., Peterson, M.L., and Stoffel, W. (1956)Am. J. Clin. Nutr. 8, 499–511.Google Scholar
  43. 43.
    Hudgins, L.C., Hirsch, J., and Emken, E.A. (1991)Am. J. Clin. Nutr. 53, 474–482.PubMedGoogle Scholar
  44. 44.
    Emken, E.A., Rohwedder, W.K., Adlof, R.O., Dejarlis, W.J., and Gully, R.M. (1985)Biochem. Biophys. Acta. 836, 233–245.PubMedGoogle Scholar
  45. 45.
    Wood, R. (1979) inGeometrical and Positional Fatty Acid Isomers (Emken, E.A., and Dutton, H.J., eds.) pp. 213–276, American Oil Chemists' Society, Champaign.Google Scholar
  46. 46.
    Reichwald-Hacker, I., Grosse-Oetringhaus, S., Kiewitt, I., and Mukherjee, K.D. (1979)Biochim. Biophys. Acta. 575, 327–334.PubMedGoogle Scholar
  47. 47.
    Ackman, R.G., Hooper, S.N., and Hooper, D.L. (1974)J. Am. Oil. Chem. Soc. 51, 42–49.Google Scholar
  48. 48.
    Wolf, R.L. (1992)J. Am. Oil. Chem. Soc. 69, 106–110.Google Scholar
  49. 49.
    Chen, Z.Y., Pelletier, G., Hollywood, R., and Ratnayake, W.M.N. (1994)INFORM 5, 473.Google Scholar
  50. 50.
    Grandgirard, A., Bourre, J.M., Julliard, F., Homayoun, P., Dumont, O., Piciotti, M., and Sebedio, J.L. (1994)Lipids 29, 251–258.PubMedGoogle Scholar
  51. 51.
    Chardigny, J.M., Bonhomme, B., Sebedio, J.L., Juaneda, P., Doly, M., and Grandgirard, A. (1994)INFORM 5, 472.Google Scholar
  52. 52.
    Applewhite, T.H., and Hunter, J.E. (1992)Lipids 27, 486.Google Scholar
  53. 53.
    Chardigny, J.M., Sebedio, J.L., Juaneda, P., Vatele, J.M., and Grandgirard, A. (1993)Nutr. Res. 13, 1105–1111.CrossRefGoogle Scholar
  54. 54.
    Pettersen, J., and Opstvedt, J. (1989)Lipids 24, 616–624.PubMedCrossRefGoogle Scholar
  55. 55.
    Moore, C.E., and Dhopeshwarkar, G.A. (1980)Lipids 15, 1023–1028.PubMedCrossRefGoogle Scholar
  56. 56.
    Rastogi, B.K., Fryer, B.A., Allee, F., and Fryer, H.C. (1980)Nutr. Rep. Inter. 22, 925–933.Google Scholar

Copyright information

© American Oil Chemists’ Society 1995

Authors and Affiliations

  • Z. -Y. Chen
    • 1
  • G. Pelletier
    • 1
  • R. Hollywood
    • 1
  • W. M. N. Ratnayake
    • 1
  1. 1.Nutrition Research Division, Food Directorate, Health Protection BranchHealth CanadaOttawaCanada

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