Lipids

, Volume 30, Issue 12, pp 1139–1143

Effect of tocotrienols on the growth of a human breast cancer cell line in culture

  • Kalanithi Nesaretnam
  • Najla Guthrie
  • Ann F. Chambers
  • Kenneth K. Carroll
Symposium

Abstract

The tocotrienol-rich fraction (TRF) of palm oil consists of tocotrienols and some α-tocopherol (α-T). Tocotrienols are a form of vitamin E having an unsaturated side-chain, rather than the saturated side-chain of the more common tocopherols. Because palm oil has been shown not to promote chemically-induced mammary carcinogenesis, we tested effects of TRF and α-T on the proliferation, growth, and plating efficiency (PE) of MDA-MB-435 estrogen-receptor-negative human breast cancer cells. TRF inhibited the proliferation of these cells with a concentration required to inhibit cell proliferation by 50% of 180 μg/mL, whereas α-T had no effect at concentrations up to 1000 μg/mL as measured by incorporation of [3H]thymidine. The effects of TRF and α-T also were tested in longer-term growth experiments, using concentrations of 180 and 500 μg/mL. We found that TRF inhibited the growth of these cells by 50%, whereas α-T did not. Their effect on the ability of these cells to form colonies also was studied, and it was found that TRF inhibited PE, whereas α-T had no effect. These results suggest that the inhibition is due to the presence of tocotrienols in TRF rather than α-T.

Abbreviations

α-T

α-tocopherol

DMBA

7,12-dimethylbenz(a)anthracene

MTT

3,(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide

PE

plating efficiency

TRF

tocotrienol-rich fraction

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Copyright information

© American Oil Chemists’ Society 1995

Authors and Affiliations

  • Kalanithi Nesaretnam
    • 1
  • Najla Guthrie
    • 2
  • Ann F. Chambers
    • 3
  • Kenneth K. Carroll
    • 2
  1. 1.Palm Oil Research Institute of MalaysiaKuala LumpurMalaysia
  2. 2.University of Western OntarioLondon
  3. 3.London Regional Cancer Research CentreLondonCanada

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