Lipids

, Volume 28, Issue 7, pp 637–643 | Cite as

Triacylglycerol structure of human colostrum and mature milk

  • Jean-Charles Martin
  • Philippe Bougnoux
  • Jean-Michel Antoine
  • Monique Lanson
  • Charles Couet
Article

Abstract

Because triacylglycerol (TAG) structure influences the metabolic fate of its component fatty acids, we have examined human colostrum and mature milk TAG with particular attention to the location of the very long chain polyunsaturated fatty acid on the glycerol backbone. The analysis was based on the formation of various diacylglycerol species from human milk TAG upon chemical (Grignard degradation) or enzymatic degradation. The structure of the TAG was subsequently deduced from data obtained by gas chromatographic analysis of the fatty acid methyl esters in the diacylglycerol subfractions. The highly specific TAG structure observed was identical in mature milk and colostrum. The three major fatty acids (oleic, palmitic and linoleic acids) each showed a specific preference for a particular position within milk TAG: oleic acid for thesn-1 position, palmitic acid for thesn-2 position and linoleic acid for thesn-3 position. Linoleic and α-linolenic acids exhibited the same pattern of distribution and they were both found primarily in thesn-3 (50%) andsn-1 (30%) positions. Their longer chain analogs, arachidonic and docosahexaenoic acids, were located in thesn-2 andsn-3 positions. These results show that polyunsaturated fatty acids are distributed within the TAG molecule of human milk in a highly specific fashion, and that in the first month of lactation the maturation of the mammary gland does not affect the milk TAG structure.

Abbreviations

BHT

butylated hydroxytoluene

DHA

docosahexaenoic acid

GPC

glycerophosphocholine

PUFA

polyunsaturated fatty acids

TAG

triacylglycerol

TLC

thin-layer chromatography

VLC-PUFA

very long chain polyunsaturated fatty acids

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Copyright information

© American Oil Chemists’ Society 1993

Authors and Affiliations

  • Jean-Charles Martin
    • 1
  • Philippe Bougnoux
    • 2
  • Jean-Michel Antoine
    • 3
  • Monique Lanson
    • 2
  • Charles Couet
    • 1
  1. 1.Laboratoire de Nutrition et Clinique Médicale A, Faculté de MédecineUniversité François RabelaisTours, cedexFrance
  2. 2.Laboratoire de Biologie des TumeursFaculté de MédcineTours
  3. 3.Direction ScientifiqueBSNParis

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