Chromatographia

, Volume 52, Issue 7–8, pp 459–464 | Cite as

Comparative separation of biologically active components inRhizoma chuanxiong by affinity chromatography with α1-acid glycoprotein and human serum albumin as stationary phasesglycoprotein and human serum albumin as stationary phases

  • H. Wang
  • H. Zou
  • J. Ni
  • B. Guo
Originals Column Liquid Chromatography
Received:
Revised:
Accepted:

Summary

Affinity chromatography with α1-acid glycoprotein (AGP) and human serum albumin (HSA) stationary phases was applied to screen and analyze the biologically active components ofRhizoma chuanxiong. Five major peaks and a number of small peaks were resolved based on their affinity for AGP and HSA, respectively, and three of them, identified as ferulic acid, chuanxiongzine and ligustilide via standard compounds, are regarded as effective components. The effects of acetonitrile concentration, pH, inorganic salt concentration and temperature on the retention behaviors of five major components on the two stationary phases were also investigated. It was observed that hydrophobicity is the major contributor to retention on both stationary phases, and ferulic acid has a weak electrostatic interaction with HSA. It demonstrated that the chromatograms ofRhizoma chuanxiong on the two stationary phases have concise and different fingerprinting characteristics. The amount of ferulic acid, chuanxiongzine and ligustilide inRhizoma chuanxiong determined using the AGP column are as much as 0.064%, 0.021% and 2.00% by weight.

Key Words

Column liquid chromatography α1-Acid glycoprotein Human serum albumin Rhizoma chuanxiong 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • H. Wang
    • 1
  • H. Zou
    • 1
  • J. Ni
    • 1
  • B. Guo
    • 2
  1. 1.National Chromatographic R&A Center, Dalian Institute of Chem. Phys.Chinese Academy of SciencesDalianP.R. China
  2. 2.Department of ChemistryCleveland State UniversityUSA

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