, Volume 24, Issue 4, pp 266–270 | Cite as

Fish oil prevents change in arachidonic acid and cholesterol content in rat caused by dietary cholesterol

  • M. L. Garg
  • A. Wierzbicki
  • M. Keelan
  • A. B. R. Thomson
  • M. T. Clandinin


Rats were fed diets high in either saturated fat (beef tallow) or α-linolenic acid (linseed oil) or eicosapentaenoic and docosahexaenoic acids (fish oil) with or without 2% cholesterol supplementation. Consumption of linseed oil and fish oil diets for 28 days lowered arachidonic acid content of plasma, liver and heart phospholipids. Addition of 2% cholesterol to diets containing beef tallow or linseed oil lowered 20∶4ω6 levels but failed to reduce 20∶4ω6 levels when fed in combination with fish oil. Feeding ω3 fatty acids lowered plasma cholesterol levels. Addition of 2% cholesterol to the beef tallow or linseed oil diet increased plasma cholesterol concentrations but not when fish oil was fed. Feeding the fish oil diet reduced the cholesterol content of liver, whereas feeding the linseed oil diet did not. Dietary cholesterol supplementation elevated the cholesterol concentration in liver in the order: linseed oil > beef tallow > fish oil (8.6-, 5.5-, 2.6-fold, respectively). Feeding fish oil and cholesterol apparently reduced 20∶4ω6 levels in plasma and tissue lipids. Fish oil accentuates the 20∶4ω6 lowering effect of dietary cholesterol and appears to prevent accumulation of cholesterol in plasma and tissue lipids under a high dietary load of cholesterol.


Cholesterol Cholesterol Content Dietary Cholesterol Beef Tallow Common Superscript 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



acyl coenzyme


cholesterol acyltransferase


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Copyright information

© American Oil Chemists’ Society 1989

Authors and Affiliations

  • M. L. Garg
    • 1
  • A. Wierzbicki
    • 1
  • M. Keelan
    • 1
  • A. B. R. Thomson
    • 1
  • M. T. Clandinin
    • 1
  1. 1.Nutrition and Metabolism Research Group, Department of Foods & Nutrition and Medicine, Division of GastroenterologyUniversity of AlbertaEdmontonCanada

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