Lipids

, Volume 24, Issue 7, pp 652–655 | Cite as

HPLC analysis of desmosterol, 7-dehydrocholesterol, and cholesterol

  • Edward H. Goh
  • Scott M. Colles
  • Kimberly D. Otte
Methods

Abstract

A simple and sensitive method to analyzed mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described. The method involves the oxidative conversion of the sterols with cholesterol oxidase, followed by high performance liquid chromatographic (HPLC) analysis. A C18 reversed phase column (3 μm, 75×4.6 mm) and a mixture of methanol and acetonitrile (1∶1, v/v) at a rate of 1 ml/min are used to separate the sterols. The eluted sterols are quantified by measuring UV absoprtion at 240 nm. As little as 10 pmoles of sterol can be quantified under these conditions.

Abbreviations

HPLC

high performance liquid chromatography

MV

millivolt

MVPH

millivolt setting of input selector and peak height at that particular input setting

UV

ultraviolet

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Copyright information

© American Oil Chemists’ Society 1989

Authors and Affiliations

  • Edward H. Goh
    • 1
  • Scott M. Colles
    • 1
  • Kimberly D. Otte
    • 1
  1. 1.Medical Sciences ProgramIndiana University School of MedicineBloomingtonUSA

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