, Volume 20, Issue 7, pp 425–432 | Cite as

Effect of brominated vegetable oils on heart lipid metabolism

  • Yolanda B. Lombardo
  • Adriana Chicco
  • María Z. Basílico
  • Claudio Bernal
  • Raul Gutman


Normal rats fed for 105 days on an experimental diet made up of standard laboratory chow supplemented with 0.5% of a mixture of brominated sunflower-olive oil (BVO) developed a significant increase in the triacylglycerol content of the heart, liver and soleus muscle compared to controls. In addition, BVO-treated rats had a decrease in plasma levels of triacylglycerol and total and HDL cholesterol. Plasma fatty acid levels and plasma post-heparin lipolytic activities, such as H-TGL, LPL, T-TGL and MGH were similar to those of control animals fed the standard chow alone.

Heart PDHa (active portion of pyruvate dehydrogenase) was dramatically decreased in the BVO-fed rats. A faster rate of spontaneous lipolysis was recorded in the isolated perfused preparation of hearts from the experimental animals. The addition of 10−7 M of glucagon to the perfusate, however, revealed a lipolytic effect comparable to the one observed in the control rats. In summary, our findings of normal fatty acids and low triacylglycerol plasma levels associated with normal activities of the various PHLA (post-heparin lipolytic activity) enzymes suggest that accumulation of triacylglycerol in heart muscle may not be explained essentially in terms of an elevated uptake and/or increased delivery of plasma fatty acids or plasma triacylglycerol. A decreased in situ catabolism of tissue triacylglycerol also appears unlikely because the spontaneous as well as the glucagon induced lipolysis in the heart both were found to be unimpaired.

Our results suggest that the mechanisms involved in the toxicologic effects of a BVO diet on heart lipid metabolism could be exerted mainly at the level of triacylglycerol biosynthesis rather than a derangement in some known step of their catabolic pathway. Additional studies are necessary to clarify this matter.


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  1. 1.
    Munro, I.C., Salem, F.A., Goodman, T., and Hasnain, S.H. (1971) Toxicol. Appl. Pharmacol. 19, 62–70.PubMedCrossRefGoogle Scholar
  2. 2.
    Munro, I.C., Hand, B., Middleton, E.J., Heggtveit, H.A., and Grice, H.C. (1972) Toxicol. Appl. Pharmacol. 22, 432–439.CrossRefGoogle Scholar
  3. 3.
    Munro, I.C., Hasnain, S., Salem, F.A., Goodman, T., Grice, H.C., and Heggtveit, H.A. (1972) in Myocardiology. Recent Advances in Studies on Cardiac Structure and Metabolism (Bajusz, E., and Rona, G., eds.) Vol. 1, pp. 588–595, University Park Press, Baltimore, London.Google Scholar
  4. 4.
    Jones, B.A., Tinsley, I.J., and Lowry, R.R. (1983) Lipids 18, 319–326.PubMedGoogle Scholar
  5. 5.
    Jones, B.A., Tinsley, I.J., and Lowry, R.R. (1983) Lipids 18, 327–334.PubMedGoogle Scholar
  6. 6.
    Shipp, J.C., Menahan, L.A., Crass, M.F. III, and Chaudhuri, S.N. (1973) Recent Adv. Studies Card. Struct. Metab. 3, 179–204.Google Scholar
  7. 7.
    Gartner, S.L., and Vahouny, G.V. (1972) Am. J. Physiol. 222, 1121–1124.PubMedGoogle Scholar
  8. 8.
    Hrön, W.T., Jesmok, G.J., Lombardo, Y.B., Menahan, L.A., and Lech, J.J. (1977) J. Mol. Cell. Cardiol. 9, 733–748.PubMedCrossRefGoogle Scholar
  9. 9.
    Lombardo, Y.B., Chicco, A., Mocchiutti, N., Rodi, M.A., Nusimovich, B., and Gutman, R. (1983) Horm. Metab. Res. 15, 69–76.PubMedGoogle Scholar
  10. 10.
    Wollenberger, A., Ristau, O., and Schoffa, G. (1960) Pflügers Arch. Gesamie Physiol. Mexachen Piere 270, 399–412.CrossRefGoogle Scholar
  11. 11.
    Krauss, R.M., Windmueller, H.G., Levy, R.I., and Fredrickson, D.S. (1973) J. Lipid Res. 14, 286–295.PubMedGoogle Scholar
  12. 12.
    Basilico, M.Z., Gutman, R., Yommi, M.R., Francone, O., and Lombardo, Y.B. (1983) Biomed. Pharmacol. 37, 36–41.Google Scholar
  13. 13.
    Gutman, R., Basilico, M.Z., Chicco, A., Rodi, M.A., Nusimovich, B., and Lombardo, Y.B., Horm. Metabol. Res., in press.Google Scholar
  14. 14.
    Vogel, W.C., Brunzell, J.D., and Bierman, E.L.A. (1971) Lipids 6, 805–814.PubMedCrossRefGoogle Scholar
  15. 15.
    Basilico, M.Z., Castro, G., Nusimovich, B., and Lombardo, Y.B. (1980) Biomedicine 33, 213–216.PubMedGoogle Scholar
  16. 16.
    Wieland, O., Siess, E., Schulze-Wethmar, F.H., Funcke, H.G.V., and Winton, B. (1971) Arch. Biochem. Biophys. 143, 593–601.PubMedCrossRefGoogle Scholar
  17. 17.
    Wieland, O.H., Patzelt, C., and Loffler, G. (1972) Eur. J. Biochem. 26, 426–433.PubMedCrossRefGoogle Scholar
  18. 18.
    Lombardo, Y.B., and Menahan, L.A. (1978) Life Sci. 22, 1033–1042.PubMedCrossRefGoogle Scholar
  19. 19.
    Laurell, S. (1966) Scand. J. Clin. Lab. Invest. 18, 668–672.PubMedGoogle Scholar
  20. 20.
    Leffler, H.H. (1959) Am. J. Clin. Pathol. 31, 310–314.PubMedGoogle Scholar
  21. 21.
    Bachorik, P.S., Wood, P.D., and Karlsson, L. (1976) Clin. Chem. 22, 1828–1832.PubMedGoogle Scholar
  22. 22.
    Duncombe, W.G. (1963) Biochem. J. 88, 7–10.PubMedGoogle Scholar
  23. 23.
    Hüijing, F. (1970) Clin. Chim. Acta 30, 567–572.PubMedCrossRefGoogle Scholar
  24. 24.
    Richard, G.M. (1974) Anal. Biochem. 57, 369–376.CrossRefGoogle Scholar
  25. 25.
    Lowry, O.H., Rosebrough, M.J., Farr, A.L., and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
  26. 26.
    Davidson, M.B., and Karjala, J. (1970) J. Lipid Res. 11, 609–612.PubMedGoogle Scholar
  27. 27.
    Snedecor, G.W.P. (1956) Statistical Methods Applied to Experiments in Agriculture and Biology, pp. 291–292, The Iowa State University Press, Ames, IA.Google Scholar
  28. 28.
    Denton, R.M., Randle, P.J., Bridges, B.J., Cooper, R.H., Kerbly, A.L., Pask, H.T., Severson, D.L., Stansble, D., and Whitehouse, S. (1975) Mol. Cell. Biochem. 9, 27–53.PubMedCrossRefGoogle Scholar
  29. 29.
    Wieland, O.H., Siess, E.A., Weiss, L., Löffler, G., Patzelt, C., Portenhauser, R., Hartmann, V., and Schirmann, A. (1973) Symp. Soc. Exp. Biol. 27, 371–400.PubMedGoogle Scholar
  30. 30.
    Wieland, O., Funcke, H.V., and Löffler, G. (1971) FEBS Lett. 15, 295–298.PubMedCrossRefGoogle Scholar
  31. 31.
    Kerbey, A.L., Randle, P.J., Cooper, R.H., Whitehouse, S., Pask, H.T., and Denton, R.M. (1976) Biochem. J. 154, 327–348.PubMedGoogle Scholar
  32. 32.
    Cornblath, M., Randle, P.J., Parmeggiani, A., and Morgan, H.E. (1963) J. Biol. Chem. 238, 1592–1597.PubMedGoogle Scholar
  33. 33.
    Lombardo, Y.B., Gutman, R., Yommi, M.R., Nusimovich, B., and Basílico, M.Z. (1982) Diabetologia 23, 183–184.Google Scholar
  34. 34.
    Nikkila, E.A., Huttunen, J.K., and Ehnholm, C. (1977) Metabolism 26, 179–186.PubMedCrossRefGoogle Scholar
  35. 35.
    Hollister, L.E., Overall, J.E., and Snow, H. (1976) Am. J. Clin. Nutr. 20, 777–782.Google Scholar
  36. 36.
    Albrink, M.J. (1962) Arch. Intern. Med. 109, 345–351.PubMedGoogle Scholar
  37. 37.
    Stam, H., and Hulsmann, W.C. (1981) Ann. Nutr. Metab. 25, 11–19.PubMedCrossRefGoogle Scholar
  38. 38.
    Murthy, V.A., Bauman, M.D., and Shipp, J. (1983) Diabetes 32, 718–722.PubMedGoogle Scholar
  39. 39.
    Murthy, V.A., and Shipp, J. (1980) J. Mol. Cell. Cardiol. 12, 299–309.PubMedCrossRefGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1985

Authors and Affiliations

  • Yolanda B. Lombardo
    • 1
  • Adriana Chicco
    • 1
  • María Z. Basílico
    • 1
  • Claudio Bernal
    • 1
  • Raul Gutman
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of LitoralSanta FeArgentina
  2. 2.Department of Experimental MedicineHospitale ItalianoBuenos AiresArgentina

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