Lipids

, Volume 20, Issue 4, pp 227–233

Effects of different dietary intake of essential fatty acids on C20∶3ω6 and C20∶4ω6 serum levels in human adults

  • M. Lasserre
  • F. Mendy
  • D. Spielmann
  • B. Jacotot
Article

Abstract

Four diets which differed in fatty acid composition were provided for five months each to a group of 24 healthy nun volunteers. The diets contained 54% carbohydrates, 16% proteins and 30% lipids. One-third of the lipid part remained unchanged during the whole study, and two-thirds were modified during each period. For this latter portion, one of the following dietary fats was used: sunflower oil, peanut oil, low erucic acid rapeseed (LEAR) oil or milk fats. This procedure allowed an evaluation of the effects of various amounts of dietary linoleic acid (C18∶2ω6) and alpha-linolenic acid (C18∶3ω3) on the serum level of their metabolites. A diet providing a large amount of linoleic acid (14% of the total caloric intake) resulted in low levels of dihomo-gamma-linolenic acid (C20∶3ω6) and arachidonic acid (C20∶4ω6) in serum phospholipids and cholesteryl esters. A diet providing a small amount of linoleic acid (0.6% to 1.3% of the total caloric intake) induced high levels of ω6 fatty acid derivatives. Intermediate serum levels of C20∶3ω6 and C20∶4ω6 were found with a linoleic acid supply of about 6.5% of the total caloric intake. Serum levels of ω6 metabolites were not different after two diets providing a similar supply of C18∶2ω6 (4.5% to 6.5% of the total caloric intake), although in one of them the supply of C18∶3ω3 was higher (1.5% for LEAR oil versus 0.13% for peanut oil).

Under our experimental conditions (healthy human adults fed on a normo-caloric diet with 30% lipids), we tried to determine PUFA (linoleic and linolenic acid) allowances which should be recommended for adults. The aim of the study was to obtain a hypocholesterolemic or normocholesterolemic effect while keeping normal 20∶3ω6 and 20∶4ω6 serum levels which would evidence a normal linoleic acid metabolism. The amounts recommended are: linoleic acid 5 to 6% of the total calories; alpha-linolenic acid 0.5 to 1% of the total calories.

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References

  1. 1.
    Burr, G.O., and Burr, M.M. (1930) J. Biol. Chem. 86, 269–282.Google Scholar
  2. 2.
    Holman, R.T. (1971) Prog. Chem. Fats Other Lipids 9, 279–348.Google Scholar
  3. 3.
    FAO study, Alimentation, Nutrition No. 3 (1977) FAO/OMS Rome.Google Scholar
  4. 4.
    Holmar, R.T. (1976) in Lipids (Paoletti, R., Porcellati, G., and Jacini, G., eds.) Vol. 1, Raven Press, New York.Google Scholar
  5. 5.
    Vergroesen, A.J., De Deckere, E.A.M., Ten Hoor, F., Hornstra, G., and Houtsmuller, U.M.M. (1975) in The Essential Fatty Acids (Hardkins, W.W., ed.) Nutrition Society of Canada, Ontario.Google Scholar
  6. 6.
    Mendy, F., Hirtz, J., Berrett, R., Rio, B., and Rossier, A. (1968) Ann. Nutr. Alim. 22, 267–285.Google Scholar
  7. 7.
    Committee on Dietary Allowances (1980) Recommended Dietary Allowances, National Academy of Sciences, Washington, D.C.Google Scholar
  8. 8.
    Holman, R.T., Johnson, S.B., and Hatch, T.F. (1982) Am. J. Clin. Nutr. 35, 613–617.Google Scholar
  9. 9.
    Lamptey, M.S., and Walker, B.L. (1976) Nutrition 106, 85–93.Google Scholar
  10. 10.
    Bang, H.O., and Dyerberg, J. (1980) Advanced Nutrition Research (Chappeer, H.H., ed.) Vol. 3, pp. 1–22, Plenum Press, New York.Google Scholar
  11. 11.
    Jacotot, B. (1979) Rev. Franc. Corps Gras 26, 171–174.Google Scholar
  12. 12.
    Enholm, L., Huttienen, J.K., Pieterren, P., Luno, V., Mutanen, M., Kostiainen, E., Pikkarainen, J., Dougherty, R., Icano, S., and Prosbky, P. (1982) New Engl. J. Med. 307, 850–855.CrossRefGoogle Scholar
  13. 13.
    Brenner, R.R., and Puluffo, R.O. (1966) J. Biol. Chem. 241, 5213–5219.PubMedGoogle Scholar
  14. 14.
    Baudet, M.F., Dachet, C., Lasserre, M., Esteva, O., and Jacotot, B. (1984) J. Lipid Res. 25, 456–468.PubMedGoogle Scholar
  15. 15.
    Etienne, G., Papin, P., and Renault, H. (1963) Ann. Biol. Clin. 10, 851–859.Google Scholar
  16. 16.
    Nishida, J. (1978) J. Med. Technol. 22, 1304.Google Scholar
  17. 17.
    Folch, J.M., Lees, M., and Sloane-Stanley, G.H. (1957) J. Biol. Chem. 226, 497–509.PubMedGoogle Scholar
  18. 18.
    Cagnazzo, G., Ros, A., and Bignardi, G. (1965) J. Chromatogr. 19, 185–189.PubMedCrossRefGoogle Scholar
  19. 19.
    Mahley, R.W. (1979) Atheroscl. Reviews 5, 1–54.Google Scholar
  20. 20.
    Hornstra, G., and Vles, R. (1978) in International Conference on Atherosclerosis (Carlson, L.A., Paoletti, R., and Sirtori, C.R., eds.) pp. 471–476. Raven Press, New York.Google Scholar
  21. 21.
    Keys, A. (1980) Seven Countries, A Multivariate Analysis of Death and Coronary Heart Disease, Harvard University Press, Cambridge, Mass.Google Scholar
  22. 22.
    Rasenen, L., Wilska, M., and Kantera, R.L. (1978) Amer. J. Clin. Nutrit. 31, 1050–1056.Google Scholar
  23. 23.
    Holman, R.T., Smythe, L., and Johnson, S. (1979) Amer. J. Clin. Nutrit. 32, 2390–2399.Google Scholar
  24. 24.
    Zumlanski, S., Budzynska-Topolowska, J., and Razanek, J. (1980) Ann. Nutrit. Alimen. 34, 668–678.Google Scholar
  25. 25.
    Hansen, A.E., Wiese, H.F., Adam, D.J.D., Boelse, A.N., Haggard, M.E., Davis, J., Newson, W.T., and Pesut, L. (1964) Amer. J. Clin. Nutrit. 15, 11–19.Google Scholar
  26. 26.
    Friedman, Z., Seyberth, H., Frölich, J., and Oates, J. (1980) Adv. Prostaglandin Thromboxane Res. 8, 1799–1805.PubMedGoogle Scholar
  27. 27.
    Iacono, J.M., Mueller, J.F., and Zellner, D.C. (1965) Amer. J. Clin. Nutrit. 16, 165–172.Google Scholar
  28. 28.
    Nouvelot, A., Dewailly, P., Fruchart, J.C., Jaillard, J., and Sezille, G. (1975) Nutrit. Metabol. 19, 307–317.CrossRefGoogle Scholar
  29. 29.
    Agradi, E., Termoli, E., Colombo, C., and Galli, C. (1978) Prostaglandins 16, 973–984.PubMedCrossRefGoogle Scholar
  30. 30.
    Dupont, J., Mathias, M.M., and Connally, P.T. (1978) Fed. Proc. 37, 445 (Abstract).Google Scholar
  31. 31.
    Ten Hoor, F., De Deckere, E.A.M., Haddeman, E., Hornstra, G., and Quadt, J.F.A. (1980) Adv. Prostaglandin Thromboxane Res. 8, 1771–1781.PubMedGoogle Scholar
  32. 32.
    De Deckere, E.A.M., Nugteren, D.H., and Ten Hoor, F., (1979) Prostaglandins 17, 947–955.PubMedCrossRefGoogle Scholar
  33. 33.
    Galli, C., Agradi, E., Petroni, A., and Socini, A. (1980) Acta Med. Scand. Suppl. 642, 171–179.Google Scholar
  34. 34.
    Galli, C., Garadi, E., Petroni, A., and Tremoli, E. (1981) Lipids 16, 165–172.PubMedGoogle Scholar
  35. 35.
    Brenner, R.R. (1977) Adv. Exper. Med. Biol. 83, 85–101.CrossRefGoogle Scholar
  36. 36.
    Morhauer, H., and Holman, R.T. (1963) J. Lipid Res. 4, 151–159.Google Scholar

Copyright information

© American Oil Chemists’ Society 1985

Authors and Affiliations

  • M. Lasserre
    • 1
  • F. Mendy
    • 2
  • D. Spielmann
    • 2
  • B. Jacotot
    • 1
  1. 1.INSERM U 32, Hôpital Henri-MondorCréteil CedexFrance
  2. 2.Unité de Recherches NutritionLaboratoires SOPHARGA/ROUSSEL UCLAFPUTEAUXFrance

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