, Volume 22, Issue 9, pp 667–668 | Cite as

Isolation and quantitation of lectins from vegetable oils

  • David M. Klurfeld
  • David Kritchevsky


The factor(s) responsible for the unexplained atherogenicity of peanut oil remain to be elucidated. To this end, we developed a technique to determine if lectin was present in the oil and to quantitate its concentration. This technique was applied to other vegetable oils including corn, soybean, and sunflower. Crude, unprocessed corn and soybean oils were also analyzed for lectin content. The crude oils contained from 858 to 2983 μg lectin per kg, while the refined oils contained 24 to 55 μg/kg of biologically active lectin. The identities of the isolated lectins were confirmed by electrophoresis on SDS-polyacrylamide gels. The biological significance of the presence of lectin in these oils remains to be determined.


Arterial Smooth Muscle Cell Peanut Lectin Lectin Content Soybean Lectin Rabbit Aortic Smooth Muscle Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



phosphate-buffered saline


  1. 1.
    Kritchevsky, D., Tepper, S.A., Scott, D.A., Klurfeld, D.M., Vesselinovitch, D., and Wissler, R.W. (1981)Atherosclerosis 38, 291–299.PubMedCrossRefGoogle Scholar
  2. 2.
    Kritchevsky, D., Davidson, L.M., Weight, M., Kriek, N.P.J., and duPlessis, J.P. (1982)Atherosclerosis 42, 53–58.PubMedCrossRefGoogle Scholar
  3. 3.
    Kritchevsky, D., Tepper, S.A., Klurfeld, D.M., Vesselinovitch, D., and Wissler, R.W. (1984)Atherosclerosis 50, 253–259.PubMedCrossRefGoogle Scholar
  4. 4.
    Tso, P., Pinkston, G., Klurfeld, D.M., and Kritchevsky, D. (1984)Lipids 19, 11–16.PubMedCrossRefGoogle Scholar
  5. 5.
    Nachbar, M.S., and Oppenheim, J.D. (1980)Am. J. Clin. Nutr. 33, 2338–2345.PubMedGoogle Scholar
  6. 6.
    Bradford, M. (1976)Anal. Biochem. 72, 248–251.PubMedCrossRefGoogle Scholar
  7. 7.
    Lotan, R., Skutelsky, E., Danon, D., and Sharon, N. (1975)J. Biol. Chem. 250, 8518–8523.PubMedGoogle Scholar
  8. 8.
    Weber, K., and Osborne, M. (1969)J. Biol. Chem. 244, 4406–4412.PubMedGoogle Scholar
  9. 9.
    Lotan, R., Cacan, R., Debray, H., Carter, W.G., and Sharon, N. (1975)FEBS Lett. 57, 100–103.PubMedCrossRefGoogle Scholar
  10. 10.
    Woods, A., Hunter, N., Sequeira, L., and Kelman, A. (1979)Plant Physiol. 63, 134.Google Scholar
  11. 11.
    Lis, H., Sela, B.-A., Sachs, L., and Sharon, N. (1970)Biochim. Biophys. Acta 211, 582–585.PubMedCrossRefGoogle Scholar
  12. 12.
    Merck Index, 9th Ed. (1976) Merck & Co., Rahway, NJ.Google Scholar
  13. 13.
    Porras, O., Carlsson, B., Fallstrom, S.P., and Hanson, L.A. (1985)Int. Archs. Allergy Appl. Immunol. 78, 30–32.Google Scholar
  14. 14.
    Scebat, L., Groult, N., Peuchmaurd, M., and Renais, J. (1984)Atherosclerosis 51, 269–280.PubMedCrossRefGoogle Scholar
  15. 15.
    Nag, S. (1985)Lab. Invest. 52, 553–558.PubMedGoogle Scholar
  16. 16.
    Davis, H.R., and Glagov, S. (1986)Atherosclerosis 61, 193–203.PubMedCrossRefGoogle Scholar
  17. 17.
    Whatley, R., Ng, S.K.-C., Rogers, J., McMurray, W.C., and Sanwal, B.D. (1976)Biochem. Biophys. Res. Commun. 70, 180–185.PubMedCrossRefGoogle Scholar
  18. 18.
    McEvoy, F.A., and Ellis, D.E. (1977)Biochem. Soc. Trans. 5, 1719–1721.PubMedGoogle Scholar
  19. 19.
    Fowler, S., Shio, H., and Haley, N.J. (1979)Lab. Invest. 41, 372–378.PubMedGoogle Scholar
  20. 20.
    Klurfeld, D.M. (1985)Arch. Pathol. Lab. Med. 109, 445–449.PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1987

Authors and Affiliations

  • David M. Klurfeld
    • 1
  • David Kritchevsky
    • 1
  1. 1.The Wistar InstitutePhiladelphia

Personalised recommendations