Lipids

, Volume 22, Issue 9, pp 667–668

Isolation and quantitation of lectins from vegetable oils

  • David M. Klurfeld
  • David Kritchevsky
Communications

Abstract

The factor(s) responsible for the unexplained atherogenicity of peanut oil remain to be elucidated. To this end, we developed a technique to determine if lectin was present in the oil and to quantitate its concentration. This technique was applied to other vegetable oils including corn, soybean, and sunflower. Crude, unprocessed corn and soybean oils were also analyzed for lectin content. The crude oils contained from 858 to 2983 μg lectin per kg, while the refined oils contained 24 to 55 μg/kg of biologically active lectin. The identities of the isolated lectins were confirmed by electrophoresis on SDS-polyacrylamide gels. The biological significance of the presence of lectin in these oils remains to be determined.

Abbreviations

PBS

phosphate-buffered saline

Copyright information

© American Oil Chemists’ Society 1987

Authors and Affiliations

  • David M. Klurfeld
    • 1
  • David Kritchevsky
    • 1
  1. 1.The Wistar InstitutePhiladelphia

Personalised recommendations