Plasmas and Polymers

, Volume 1, Issue 4, pp 299–326 | Cite as

Surface chemical composition and fibrinogen adsorption-retention of fluoropolymer films deposited from an RF glow discharge

  • Pietro Favia
  • Victor H. Perez-Luna
  • Thomas Boland
  • David G. Castner
  • Buddy D. Ratner
Article
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Accepted:

Abstract

Fluoropolymer films have been deposited in the glow and afterglow regions of radio frequency glow discharges fed with C2F6−H2 mixtures. Structure, growth rate, composition, and wettability of the films have been investigated by means of atomic force microscopy, electron spectroscopy for chemical analysis, secondary ion mass spectrometry, and water contact angle measurements.125I labeled baboon fibrinogen in baboon plasma has been used to study the adsorption of the protein onto the films. Protein retention, i.e., the binding affinity of the adsorbed protein, has been examined by elution with a sodium dodecyl sulfate solution. Adsorption and retention of fibrinogen were correlated using multivariate statistical methods with the wettability, the degree of film fluorination, and the CF x (1≤x≤3) group distribution of the coatings. This correlation identified the influence of each variable on the adsorption and retention of fibrinogen onto these substrates. These variables or surface properties can be easily balanced by properly tuning the experimental conditions of the glow discharge deposition process.

Key Words

Fluoropolymer films 125I labeled baboon fibrinogen sodium dodecyl sulfate solution protein adsorption glow discharge deposition 
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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Pietro Favia
    • 1
  • Victor H. Perez-Luna
    • 1
  • Thomas Boland
    • 1
  • David G. Castner
    • 1
  • Buddy D. Ratner
    • 1
  1. 1.National ESCA and Surface Analysis Center for Biomedical Problems, Department of Chemical EngineeringUniversity of WashingtonSeattle

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