Lipids

, Volume 10, Issue 7, pp 417–420 | Cite as

The incorporation of orally fed radioactive γ-linolenic acid and linoleic acid into the liver and brain lipids of suckling rats

  • A. G. Hassam
  • A. J. Sinclair
  • M. A. Cramwford
Article

Abstract

The incorporation of radioactivity from orally administered γ-linolenic acid-1-14C and linoleic acid-3H into the liver, plasma, and brain lipids of suckling rats was studied. Significantly more radioactivity from the former compound was incorporated into the liver and brain lipids 22 hr after dosing. The distribution of the radioactivity in the fatty acids of the liver and brain lipids was different for each isotope. Most of the3H was still associated with linoleic acid, whereas most of the14C was in the 20∶3 and 20∶4ω6 fractions. These results suggest that the desaturation of linoleic to γ-linolenic acid in vivo is a rate-limiting step in the conversion of linoleic to arachidonic acid.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sinclair, A.J., Lipids 10:175 (1975).PubMedGoogle Scholar
  2. 2.
    Marcet, Y.L., K. Christiansen, and R.T. Holman, Biochim. Biophys. Acta 164:25 (1968).Google Scholar
  3. 3.
    Sinclair, A.J., and M.A. Crawford, Brit. J. Nutr. 29:127 (1973).PubMedCrossRefGoogle Scholar
  4. 4.
    Sinclair, A.J., Lipids 9:809 (1974).PubMedCrossRefGoogle Scholar
  5. 5.
    Goldfine, H., and K. Block, J. Biol. Chem. 236:2596 (1961).PubMedGoogle Scholar
  6. 6.
    Snedecor, G.W. “Statistical Methods”, Fifth Edition, Iowa State College Press, Ames, Iowa, 1956.Google Scholar
  7. 7.
    Brenner, R.R., Lipids 6:567 (1971).PubMedCrossRefGoogle Scholar
  8. 8.
    Davidson, S., R. Passmore, and J.F. Brock, “Human Nutrition and Dietetics”, Fifth Edition, Churchill Livingstone, Edinburgh, Scotland, 1972, p. 64.Google Scholar
  9. 9.
    Garcia, P.T., and R.T. Holman, JAOCS 42: 1137 (1965).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1975

Authors and Affiliations

  • A. G. Hassam
    • 1
  • A. J. Sinclair
    • 1
  • M. A. Cramwford
    • 1
  1. 1.Nuffield Institute of Comparative MedicineThe Zoological Society of LondonLondonUK

Personalised recommendations