Advertisement

Lipids

, Volume 9, Issue 2, pp 121–128 | Cite as

Effect of (−)-hydroxycitrate upon the accumulation of lipid in the rat: I. Lipogenesis

  • Ann C. Sullivan
  • Joseph Triscari
  • James G. Hamilton
  • O. Neal Miller
  • Victor R. Wheatley
Symposium: Effect of Drugs on Lipid Metabolism Presented at the AOCS Spring Meeting, New Orleans

Abstract

The purpose of these investigations was to ascertain the effect of (−)-hydroxycitrate on the accumulation of lipid in the meal fed rat by examining the rates of lipogenesis after acute and chronic treatment. Oral administration of (−)-hydroxycitrate depressed significantly the in vivo lipogenic rates in a dose-dependent manner in the liver, adipose tissue, and small intestine. The hepatic inhibition was significant for the 8 hr period, when control animals demonstrated elevated rates of lipid synthesis. The kinetics of this reduction of in vivo hepatic lipogenesis were identical after acute or chronic administration of (−)-hydroxycitrate. However, in vitro rates of lipogenesis were elevated after chronic administration of (−)-hydroxycitrate for 30 days. Rats receiving (−)-hydroxycitrate consumed less food than the untreated controls; however, this decreased caloric intake was not responsible for the drug induced depression of hepatic lipogenesis, as shown by studies using pair fed rats.

Keywords

Cholesterol Synthesis Lipid Synthesis Fatty Acid Synthetase Hepatic Lipogenesis Chronic Oral Administration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lewis, Y.S., and S. Neelakantan, Phytochem. 4:619 (1965).CrossRefGoogle Scholar
  2. 2.
    Lewis, Y.S., S. Neelakantan, and C. Anjanamurthy, Curr. Sci. 3:82 (1964).Google Scholar
  3. 3.
    Lewis, Y.S., in “Methods in Enzymology,” Vol. 13, Edited by J.M. Lowenstein, Academic Press, New York, N.Y., 1969.Google Scholar
  4. 4.
    Lowenstein, J.M., in “Essays in Cell Metabolism,” Edited by W. Bartley, H.L. Kornberg, and J. R. Quayle, John Wiley and Sons, London, England, 1970.Google Scholar
  5. 5.
    Watson, J.A., M. Fang, and J.M. Lowenstein, Arch. Biochem. Biophys. 135:209 (1969).PubMedCrossRefGoogle Scholar
  6. 6.
    Sullivan, A.C., J.G. Hamilton, O.N. Miller, and V.R. Wheatley, Ibid. 150:183 (1972).PubMedCrossRefGoogle Scholar
  7. 7.
    Brunengraber, H., J.R. Sabine, M. Boutry, and J.M. Lowenstein, Ibid., 150:392 (1972).PubMedCrossRefGoogle Scholar
  8. 8.
    Barth, C., J. Hackenschmidt, H. Ullmann, and K. Decker, FEBS Letters 22:343 (1972).PubMedCrossRefGoogle Scholar
  9. 9.
    Lowenstein, J.M., J. Biol. Chem. 246:629 (1971).PubMedGoogle Scholar
  10. 10.
    Sullivan, A.C., O.N. Miller, J.S. Wittman, and J.G. Hamilton, J. Nutr. 101:265 (1971).PubMedGoogle Scholar
  11. 11.
    Jungas, R.L., Biochemistry 7:3708 (1968).PubMedCrossRefGoogle Scholar
  12. 12.
    Fain, J.N., and R.O. Scow, Amer. J. Physiol. 210:19 (1965).Google Scholar
  13. 13.
    Windmueller, H.G., and A.E. Spaeth, J. Biol. Chem. 241:2891 (1966).PubMedGoogle Scholar
  14. 14.
    Fain, J.N., and A.E. Wilhelmi Endocrinology 71:541 (1962).Google Scholar
  15. 15.
    Windmueller, H.G., and A.E. Spaeth, Arch. Biochem. Biophys. 122:362 (1967).PubMedCrossRefGoogle Scholar
  16. 16.
    Fain, J.N., R.O. Scow, E.J. Urgoiti, and S.S. Chernick, Endocrinology 77:137 (1965).CrossRefGoogle Scholar
  17. 17.
    Phillips, P.H., and E.B. Hart, J. Biol. Chem. 109:657 (1935).Google Scholar
  18. 18.
    Bligh, E.G., and W.J. Dyer, Can. J. Biochem. Physiol. 37:911 (1959).PubMedGoogle Scholar
  19. 19.
    Fang, M., and J.M. Lowenstein, Biochem. J. 105:803 (1967).PubMedGoogle Scholar
  20. 20.
    Miller, G.L., Anal. Chem. 31:964 (1959).CrossRefGoogle Scholar
  21. 21.
    Snedecor, G.W., and W.G. Cochran, in “Statistical Methods,” Iowa State University Press, Ames, Iowa, 1968.Google Scholar
  22. 22.
    Dixon, W.J., Biometrics 9:74 (1953).CrossRefGoogle Scholar
  23. 23.
    Leveille, G.A., Proc. Soc. Exp. Biol. Med., 125:85 (1967).PubMedGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1974

Authors and Affiliations

  • Ann C. Sullivan
    • 1
  • Joseph Triscari
    • 1
  • James G. Hamilton
    • 1
  • O. Neal Miller
    • 1
  • Victor R. Wheatley
    • 1
  1. 1.Department of Biochemical NutritionHoffmann-LaRoche Inc.Nutley

Personalised recommendations