, Volume 5, Issue 5, pp 494–496

Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots

  • George Rouser
  • Sidney Fleischer
  • Akira Yamamoto
Short Communications

DOI: 10.1007/BF02531316

Cite this article as:
Rouser, G., Fleischer, S. & Yamamoto, A. Lipids (1970) 5: 494. doi:10.1007/BF02531316


Separation of polar lipids by two-dimensional thin layer chromatography providing resolution of all the lipid classes commonly encountered in animal cells and a sensitive, rapid, reproducible procedure for determination of phospholipids by phosphorus analysis of spots are described. Values obtained for brain and mitochondrial inner membrane phospholipids are presented.

Copyright information

© American Oil Chemists’ Society 1969

Authors and Affiliations

  • George Rouser
    • 1
  • Sidney Fleischer
    • 2
  • Akira Yamamoto
    • 3
  1. 1.Division of NeurosciencesCity of Hope Medical CenterDuarte
  2. 2.Department of Molecular BiologyVanderbilt UniversityNashville
  3. 3.City of Hope Medical CenterDuarte

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