Archives of Microbiology

, Volume 164, Issue 6, pp 420–427 | Cite as

Ultrastructural studies of neutral lipid localisation inStreptomyces

Original Paper
Received:
Accepted:

Abstract

Triacylglycerol is accumulated byStreptomyces spp. when grown in submerged culture. Ultrastructural studies using transmission electron microscopy (TEM), staining and freeze-fracture/freeze-etch procedures, and light microscopy confirmed the accumulation of neutral lipid byS. lividans andS. coelicolor during the stationary phase and its storage within membrane-bound globular structures within the cytoplasm. There structures were of various sizes and occupied up to approximately 80% of the total cell volume at that time. There was no evidence of such material within cells examined during the early exponential phase of growth. The globules visualised by TEM were electron-transparent since they comprised lipids containing saturated fatty acids that did not react with osmium tetroxide. The globules appeared to be bounded by a single membrane.

Key words

Transmission electron microscopy Light microscopy Triacylglycerols Streptomyces Sudan black B 

Abbreviations

TAG

triacylglycerol

TEM

transmission electron microscopy

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ballio A, Barcellona S, Boniforti L (1965) The component fatty acids of lipids from someStreptomyces species. Biochem J 94:11C-13CPubMedGoogle Scholar
  2. Braña AF, Manzanal MB, Hardisson C (1982) Characterization of intracellular polysaccharides ofStreptomyces. Can J Microbiol 28:1320–1323PubMedGoogle Scholar
  3. Braña AF, Méndez C, Díaz LA, Manzanal MB, Hardisson C (1986) Glycogen and trehalose accumulation during colony development inStreptomyces antibioticus. J Gen Microbiol 132: 1319–1326PubMedGoogle Scholar
  4. Burdon KL (1946) Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. J Bacteriol 52:665–678PubMedGoogle Scholar
  5. Chater KF, Merrick MJ (1979) Streptomycetes. In: Parish JH (ed) Developmental biology of prokaryotes. University of California Press, Berkeley Los Angeles, pp 93–114Google Scholar
  6. Dawes (1985) Starvation, survival and energy reserves. In: Fletcher M, Floodgate GD (eds) Bacteria in their natural environments. Society of General Microbiology Special Publication, no 16. Academic Press, London, pp 43–79Google Scholar
  7. Daza A, Martin JF, Dominguez A, Gil JA (1989) Sporulation of several species ofStreptomyces in submerged cultures after nutritional downshift, J Gen Microbiol 235:2483–2491Google Scholar
  8. Deetz JS, Behrman EJ (1981) Reaction of osmium reagents with amino acids and proteins. Int J Pept Protein Res 17:495–500PubMedCrossRefGoogle Scholar
  9. Friend DS (1969) Cytochemical staining of multivesicular body and Golgi vesicles. J Cell Biol 41:269–279PubMedCrossRefGoogle Scholar
  10. Gale GR, McLlain HH (1963) Effect of ethambutol on cytology ofMycobacterium smegmatis. J Bacteriol 86:749–756PubMedGoogle Scholar
  11. Gurr MI (1980) The biosynthesis of triacylglycerols. In: Stumpf PK (ed) The biochemistry of plants, vol 4. Academic Press, New York London, pp 205–249Google Scholar
  12. Gurr MI, Harwood JL (1991) Lipids as energy stores. In: Lipid biochemistry, 4th edn. Chapman & Hall, London, pp 126–130Google Scholar
  13. Gurr MI, Blades J, Appleby RS, Smith CG, Robinson MP, Nichols BW (1974) Triglyceride biosynthesis and storage in whole seeds and oil bodies ofCrambe abyssinica. Eur J Biochem 43:281–290PubMedCrossRefGoogle Scholar
  14. Hayat MA (1989) Chemical fixation. In: Principles and techniques of electron microscopy: biological applications, 3rd edn. Macmillan, London, pp 42–46Google Scholar
  15. Holdsworth JE, Veenhuis M, Ratledge C (1988) Enzyme activities in oleaginous yeasts accumulating and utilizing exogenous or endogenous lipids. J Gen Microbiol 134:2907–2915PubMedGoogle Scholar
  16. Hopwood DA (1967) Genetic analysis and genome structure inStreptomyces coelicolor. Bacteriol Rev 31:373–403PubMedGoogle Scholar
  17. Jensen TE, Sicko LM (1971) Fine structure of poly-β-hydroxybutyric acid granules in a blue-green alga,Chlorogloea fritschii. J Bacteriol 106:683–686.PubMedGoogle Scholar
  18. Kannan LV, Rehacek Z (1970) Formation of poly-β-hydroxybutyrate by Actinomycetes. Indian J Biochem 7:126–129PubMedGoogle Scholar
  19. Martin JF, Demain AL (1980) Control of antibiotic synthesis. Microbiol Rev 44:230–251PubMedGoogle Scholar
  20. Olukoshi ER, Packter NM (1994) Importance of stored triacylglycerols inStreptomyces: possible carbon source for antibiotics. Microbiology 140:931–943PubMedCrossRefGoogle Scholar
  21. Packter NM, Flatman S, Lucock AJ (1985) Formation of storage lipids and actinorhodin, a phenolic antibiotic inStreptomyces coelicolor. Biochem Soc Trans 13:251–252Google Scholar
  22. Ranade N, Vining LC (1993) Accumulation of intracellular carbon reserves in relation to chloramphenicol biosynthesis byStreptomyces venezuelae. Can J Microbiol 39:377–383PubMedCrossRefGoogle Scholar
  23. Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212PubMedCrossRefGoogle Scholar
  24. Ryter A, Kellenberger E (1958) Étude au microscope électronique de plasmas contenant de l'acide désoxyribonucleique. Z Naturforsch 13B:597–605Google Scholar
  25. Shively JM (1974) Annu Rev Microbiol 28:167–187PubMedCrossRefGoogle Scholar
  26. Wildermuth H, Hopwood DA (1970) Septation during sporulation inStreptomyces coelicolor. J Gen Microbiol 60:51–59PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyThe University of LeedsLeedsUK

Personalised recommendations