Medical and Biological Engineering and Computing

, Volume 36, Issue 6, pp 821–824 | Cite as

Reconstructed human epidermis composed of keratinocytes, melanocytes and Langerhans cells

  • M. Régnier
  • A. Patwardhan
  • A. Scheynius
  • R. Schmidt
Cellular Engineering: Bioengineering of the Skin

Abstract

In addition to their basic biological interest, models of reconstructed epidermis provide useful tools for in vitro assessment of the toxicology and efficacy of new chemicals and drugs. The fact that the majority of these in vitro models are composed only of keratinocytes has excluded their use in the fields of skin pigmentation and immunology. After the successful introduction of functional melanocytes into the epidermal reconstruct, the integration of Langerhans cells remains an important challenge, particularly since after isolation of Langerhans cells from human epidermis, these cells cannot be subcultured and do not integrate into the reconstructing epidermis. The authors show that cord blood derived and CD34+ progenitors isolated from the peripheral blood give rise to residential Langerhans cells when co-seeded with normal human keratinocytes.

Keywords

Reconstructed epidermis Langerhans cells Melanocytes 

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Copyright information

© IFMBE 1998

Authors and Affiliations

  • M. Régnier
    • 1
  • A. Patwardhan
    • 2
  • A. Scheynius
    • 2
  • R. Schmidt
    • 1
  1. 1.Life Science ResearchCentre Charles ZviakClichyFrance
  2. 2.Laboratory Medicine Division of Clinical ImmunologyKarolinska Institute and HospitalStockholmSweden

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