Medical and Biological Engineering and Computing

, Volume 36, Issue 6, pp 778–790 | Cite as

Cultivation of human keratinocyte stem cells: current and future clinical applications

  • G. Pellegrini
  • S. Bondanza
  • L. Guerra
  • M. De Luca
Cellular Engineering: Bioengineering of the Skin

Abstract

Cultured human keratinocytes have a wide spectrum of clinical applications. Clinical results reported by several investigators are, however, contradictory. In this review, the authors discuss the biological and surgical issues which play a key role in the clinical outcome of cultured epidermal autografts used for the treatment of massive full-thicknes burns. The importance of cultivation of epidermal stem cells and of their transplantation onto a wound bed prepared with donor dermis is emphasised. The paper also reviews recent data showing that: (i) cultured epidermal autografts bearing melanocytes can be used for the treatment of stable vitiligo; (ii) keratinocytes isolated from other lining epithelia, such as oral, urethral and corneal epithelia, can be cultivated and grafted onto patients suffering from disabling epithelial defects; (iii) keratinocyte stem cells can be stably transduced with retroviral vectors and are therefore attractive targets for the gene therapy of genodermatoses.

Keywords

Keratinocyte Stern cell Skin Epidermis Burns Gene therapy Genodermatoses Melanocyte Vitiligo Tissue engineering 

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Copyright information

© IFMBE 1998

Authors and Affiliations

  • G. Pellegrini
    • 1
  • S. Bondanza
    • 1
  • L. Guerra
    • 1
  • M. De Luca
    • 1
  1. 1.Laboratory of Tissue Engineering, I.D.C.-IRCCSIstituto Dermopatico dell'ImmacolataPomezia, RomaItaly

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