Accumulation of phyllodulcin in sweet-leaf plants ofHydrangea serrata and its neutrality in the defence against a specialist leafmining herbivore

  • Mami Ujihara
  • Masateru Shinozaki
  • Makoto Kato
Original Paper


Among wild plants ofHydrangea serrata (Hydrangeaceae) in Japan, there are sweet plants whose leave contain a kind of isocoumarin, phyllodulcin, which happens to be 350 times as sweet as sucrose to the human tongue. In a primary beech forest in Ashu, Kyoto, the spatial distribution of sweet plants and temporal and the spatial distribution of phyllodulcin within and among plants were investigated using a high performance liquid chromatograph. The distribution of sweet plants was confined within a valley and was parapatric with non-sweet plants. A plant's characteristic phyllodulcin accumulation did not change, even when transplanted into the different habitats. The phyllodulcin content of the sweet plants varied greatly among plants, and the population mean peaked in July when the plants flowered. Within a plant, phyllodulcin content was elevated by partial defoliation. We examined the possible effect of phyllodulcin on herbivory by a specialist leafmining herbivore,Antispila hydrangifoliella (Lepidoptera: Heliozelidae). We transplanted sweet and nonsweet plants reciprocally between their original habitats, excluded attacks by parasitoids, and compared performance of the leafminer. Leafminer colonization and larval survivorship on transplanted andin situ plants was not significantly different between sites. The fact that accumulation of phyllodulcin did not augment a defensive function, at least against herbivory by the leafminer, and the sporadic distribution of phyllodulcin-accumulating plants, suggest that the genotypes synthesizing phyllodulcin emerged independently at separate localities by mutation, and that the genotypes are almost adaptively neutral in defence against the specialist herbivore.

Key words

Hydrangea chemical defence leafminer Antispila phyllodulcin sweetener 


  1. Asahina, Y. and J. Asano (1929) Über die Konstitution von Hydrageol und Phyllodulcin (1).Chem. Ber. 62: 171–181.Google Scholar
  2. Asahina, Y. and J. Asano (1930) Über die Konstitution von Hydragenol und Phyllodulcin (2. Mitteil).Chem. Ber. 63: 429–437.Google Scholar
  3. Berenbaum, M. (1983) Coumarins and caterpillars: a case for coevolution.Evolution 37: 163–179CrossRefGoogle Scholar
  4. Coleman, J. S. and C. G. Jones (1991) A phytocentric perspective of phytochemical induction by herbivores. pp. 3–45.In D. W. Tallamy and M. J. Raupp (eds.)Phytochemical induction by herbivores. John Wiley & Sons, New York.Google Scholar
  5. Edwards, P. B., W. J. Wanjura and W. V. Brown (1993) Selective herbivory by Christmas beetles in response to intraspecific variation inEucalyptus terpenoids.Oecologia 95: 551–557.Google Scholar
  6. Ehrlich, P. R. and P. H. Raven (1964) Butterflies and plants: a study in coevolution.Evolution 18: 586–608.CrossRefGoogle Scholar
  7. Feeny, P. (1976) Plant apparency and chemical defenseRec. Adv. Phytochem. 10: 1–40.Google Scholar
  8. Fritz, R. S., C. Sacchi and P. W. Price (1986) Competition versus host plant phenotype in species composition: willow sawflies.Ecology 67: 1608–1618.CrossRefGoogle Scholar
  9. Fritz, R. S. and P. W. Price (1988) Genetic variation among plants and insect community structure: willows and sawflies.Ecology 69:845–856.CrossRefGoogle Scholar
  10. Hashimoto, T., M. Tori and Y. Asakawa (1987) Three dihydroisocoumarin glucosides fromHydrangea macrophylla subsl.serrata.Phytochem. 26: 3323–3330.CrossRefGoogle Scholar
  11. Langenheim, J. H., C. L. Corvis, C. A. Macedo and W. H. Stubblebine (1986)Hymenaea andCopaifera leaf sesquiterpenes in relation to lepidopteran herbivory in southeastern Brazil.Biochem. Syst. Ecol. 14: 41–49.CrossRefGoogle Scholar
  12. Karban, R. and J. H. Myers (1989) Induced plant responses to herbivory.Annu. Rev. Ecol. Syst. 20: 331–348.CrossRefGoogle Scholar
  13. Kimura, Y. (1953) Amacha.Yakkyoku 4: 373–374 (in Japanese)Google Scholar
  14. Kuroko, H. (1982) Heliozelidae. pp 56–61.In H. Inoue, S. Sugi, H. Kuroko, S. Moriuchi and A. Kawabe (eds.),Moths of Japan. Kôdansha, Tokyo. (in Japanese).Google Scholar
  15. Nozawa, K., M. Yamada, Y. Tsuda, K. Kawai and S. Nakajima (1981) Antifungal activity of oosponol, oospolactone, phyllodulcin, hydrangenol, and some other related compounds.Chem. Pharm. Bull. 29: 2689–2691.PubMedGoogle Scholar
  16. Ohba, H. (1989) Saxifragaceae. pp. 158–176.In Y. Satake, H. Hara, S. Watari and T. Tominari (eds.)Wild flowers of Japan: Woody plants (Heibonsha, Tokyo.Google Scholar
  17. Rhoades, D. F. (1979) Evolution of plant chemical defense against herbivores. pp. 3–34.In G. A. Rosenthal and D. J. Janzen (eds.)Herbivores their interaction with secondary plant metabolites. Accademic Pres, New YorkGoogle Scholar
  18. Sturgeon, K. B. (1979) Monoterpene variation in ponderosa pine xylen resin related to western pine beetle predation.Evolution 33: 803–814.CrossRefGoogle Scholar
  19. Suzuki, H., T. Matsumoto, T. Kisaki and M. Noguchi (1981) Influences of cultural conditions on polyphenol formation and growth of Amacha cells (Hydrangea macrophylla Seringe var.Thunbergii Makino) and changes of polyphenol contents in leaves of Amacha plant during growth.Agric. Biol Chem. 45: 1067–1077.Google Scholar
  20. Tuomi, J. (1992) Toward integration of plant defence theories.Trends Ecol. Evol. 7: 365–367.CrossRefGoogle Scholar
  21. Ueno, M. and R. Mori (1931) On the activity of sweet compounds of amacha, phyllodulcin, against bacteria.Yakugaku Zasshi 51: 227–230.Google Scholar
  22. Ueno, Y. (1937) Über das Hydrangenolglykosid.Yakugaku Zasshi 57: 602–605.Google Scholar
  23. Watanabe, N., S. Watanabe, R. Nakajima, J. H. Moon, K. Shimokihara, J. Inagaki, H. Etoh, T. Asai, K. Sakata and K. Ina (1993) Formation of flower fragrance compounds from their precursors by exzymic action during flower opening.Biosci. Biotech. Biochem. 57: 1101–1107.CrossRefGoogle Scholar
  24. Yamato, M., K. Hashigaki, J. Uenishi, I. Yamakawa, N. Sato and T. Koyama (1975) Chemical structure and sweet taste of isocoumarins and related compounds. VI.Chem. Pharm. Bull. 23: 3101–3105.PubMedGoogle Scholar
  25. Yamato, M., K. Hashigaki, K. Mito and T. Koyama (1978) Chemical structure and sweet taste of isocoumarins and related compounds. X. Syntheses of sweet 5-hydroxyflavanones and related dihydrochalcones.Chem. Pharm. Bull. 26: 2321–2327.PubMedGoogle Scholar
  26. Yasuda, S. and H. Nagamasu (1995) Flora of Ashiu, Japan.Contr. Biol. Lab. Kyoto Univ. 28: 367–486.Google Scholar

Copyright information

© Society of Population Ecology 1995

Authors and Affiliations

  • Mami Ujihara
    • 1
  • Masateru Shinozaki
    • 2
  • Makoto Kato
    • 1
  1. 1.Biological Laboratory, Yoshida CollegeKyoto UniversityKyotoJapan
  2. 2.Laboratory of Applied Botany, Faculty of AgricultureKyoto UniversityKyotoJapan

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