Journal of Molecular Evolution

, Volume 32, Issue 2, pp 101–104

Pyrophosphate formation as the most efficient condensation reaction of activated nucleotides

  • Libaniel Rodriguez
  • Leslie E. Orgel

DOI: 10.1007/BF02515382

Cite this article as:
Rodriguez, L. & Orgel, L.E. J Mol Evol (1991) 32: 101. doi:10.1007/BF02515382


When an oligonucleotide primer pG10 is incubated with the nucleotide analogue 9-[3-hydroxy-2-(hydroxymethyl)prop-1-yl] guanine diphosphate (\(p\overline{\overline G} p\), I) in the presence of poly(C), addition of the monomer occurs almost exclusively at the 5′-terminal phosphate rather than the 3′-terminalcis-glycol. The implications of this finding in the context of prebiotic condensation reactions are discussed.

Key words

Pyrophosphate formation Template-directed synthesis Nucleotide analogues 

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Libaniel Rodriguez
    • 1
  • Leslie E. Orgel
    • 1
  1. 1.The Salk Institute for Biological StudiesSan DiegoUSA

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