Neurophysiology

, Volume 32, Issue 2, pp 63–69 | Cite as

Properties of the apamin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pigtaenia coli

  • A. V. Povstyan
  • A. V. Zima
  • M. I. Harhun
  • M. F. Shuba
Article
Received:

Abstract

With the help of a standard voltage-clamp technique, we investigated transmembrane ion currents in isolated smooth muscle cells of the guinea pigtaenia coli. In Ca2+-dependent K+ current, we identified and studied the properties of an apamin-sensitive voltage-independent component carried through the channels of low conductance (in many publications called small conductance,I SK(Ca)). This component did not show the temporal inactivation;I SK(Ca) was insensitive to the action of 4 mM tetraethylammonium, but was completely blocked by 500 nM of apamin. It was shown thatI SK(Ca) is very sensitive to changes in the intracellular Ca2+ concentration ([Ca2+] i ): a decrease in [Ca2+] i up to 50 nM resulted in the almost complete blockade of the current. The entry of Ca ions into a cell from the external solution through the voltage-operated Ca2+ channels of L-type was not an obligatory condition for activation ofI SK(Ca). The current-voltage relationship forI SK(Ca) had a maximum within the voltage range of +20 to +50 mV.

Keywords

smooth muscle cells Ca2+-dependent K+ channels intracellular Ca2+ concentration apamin tetraethylammonium 
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Copyright information

© Kluwer Academic/Plenum Publishers 2000

Authors and Affiliations

  • A. V. Povstyan
    • 1
  • A. V. Zima
    • 1
  • M. I. Harhun
    • 1
  • M. F. Shuba
    • 1
  1. 1.Bogomolets Institute of PhysiologyNational Academy of Sciences of UkraineKyivUkraine

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