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Journal of Plant Research

, Volume 111, Issue 3, pp 453–458 | Cite as

The promotive effects of a peptidyl plant growth factor, phytosulfokine-α, on the formation of adventitious roots and expression of a gene for a root-specific cystatin in cucumber hypocotyls

  • Seiyei Yamakawa
  • Chiyoko Sakuta
  • Yoshikatsu Matsubayashi
  • Youji Sakagami
  • Hiroshi Kamada
  • Shinobu Satoh
Original Articles

Abstract

Phytosulfokines (PSKs) were the first peptidyl growth factors from a higher plant to be characterized. They induce the proliferation in suspension culture of mesophyll cells of asparagus (Asparagus officinalis L.; Matsubayashi and Sakagami 1996b). We examined the effects of PSK-α on the formation of adventitious roots on cucumber hypocotyls. The number of roots increased 2- to 2.5-fold after treatment with PSK-α but derivatives of PSK-α were inactive in our assay system. The effect of PSK-α on the formation of adventitious roots was also examined in terms of the expression of a newly isolated gene for a root-specific cystatin of cucumber (RCC). The results indicate that PSK-α can promote organogenesis in plant, acting possibly via enhancement of the proliferation of plant cells.

Key words

Adventitious root Cystatin Cucumber Peptidyl growth factor Phytosulfokine 

Abbreviations

CM

conditioned medium

PSK

phytosulfokine

RCC

root-specific cystatin of cucumber

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Copyright information

© The Botanical Society of Japan 1998

Authors and Affiliations

  • Seiyei Yamakawa
    • 1
  • Chiyoko Sakuta
    • 1
  • Yoshikatsu Matsubayashi
    • 2
  • Youji Sakagami
    • 2
  • Hiroshi Kamada
    • 1
  • Shinobu Satoh
    • 1
  1. 1.Institute of Biological SciencesUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.Department of Biological SciencesNagoya UniversityNagoyaJapan

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