Journal of Plant Research

, Volume 110, Issue 4, pp 463–467 | Cite as

Analysis of genomic sequences encoding a sex pheromone from theClosterium peracerosum-strigosum-littorale complex

  • Bunshiro EndoEmail author
  • Tadashi Fujii
  • Yuji Kamiya
  • Hiroyuki Sekimoto
Original Articles


Genes for 19-kDa and 42-kDa subunits of protoplast-release-inducing protein (PR-IP) were cloned from theClosterium peracerosum-strigosum-littorale complex mt+ cells. Genomic sequences and transcriptional initiation sites for both genes were determined. Sequence data revealed that typical TATA boxes are located immediately upstream of both transcriptional initiation sites. Several similar sequences were observed at nearly the same location in the promoters of both genes. Based on these data, we propose some possible hypotheses regulating the expression of the PR-IP subunit genes.

Key words

Cis-element Closterium Genomic DNA Protoplast-release-inducing protein (PR-IP) Sex pheromone Transcription 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Banuett, F., 1995. Genetics ofUstilago maydis, a fungal pathogen that induces tumors in maize. Annu. Rev. Genetics29: 179–208.CrossRefGoogle Scholar
  2. Bölker, M. andKahmann, R. 1993. Sexual pheromones and mating responses in fungi. Plant Cell5: 1461–1469.PubMedCrossRefGoogle Scholar
  3. Bölker, M., Urban, M. andKahmann, R. 1992. The a mating-type locus ofUstilago maydis specifies cell signaling components. Cell68: 441–450.PubMedCrossRefGoogle Scholar
  4. Edwards, J.B.D.M., Delort, J. andMallet, J. 1991. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for doing 5′ ends of mRNAs and for constructing cDNA libraries byin vitro amplification. Nucleic Acids Res.19: 5227–5232.PubMedGoogle Scholar
  5. Ferris, P.J. andGoodenough, U.W. 1997. Mating type inChlamydomonas is specified bymid, the minus-dominance gene. Genetics146: 859–869.PubMedGoogle Scholar
  6. Hamada, J., Yoshizawa-Katoh, T. andTsunewaki, K. 1982. Genetics study on mating type genes by a new type of tetrad analysis inClosterium ehrenbergii. Bot. Mag. Tokyo95: 101–108.CrossRefGoogle Scholar
  7. Ichimura, T. 1971. Sexual cell division and conjugation-papilla formation in sexual reproduction ofClosterium strigosum.In K. Nishizawa, ed., Proc. of the 7th Int. Seaweed Symp., University of Tokyo Press, Tokyo, pp. 208–214.Google Scholar
  8. Kasai, F. andIchimura, T. 1990. A sex determining mechanism in theClosterium ehrenbergii (Chlorophyta) species complex. J. Phycol.26: 195–201.CrossRefGoogle Scholar
  9. Nojiri, T., Fujii, T. andSekimoto, H. 1995. Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallicClosterium peracerosum-strigosum-littorale complex. Plant Cell Physiol.36: 79–84.Google Scholar
  10. Sambrook, J., Fritsch, E.F. andManiatis, T. 1989. Molecular Cloning, A laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  11. Sekimoto, H., Satoh, S. andFujii, T. 1990. Biochemical and physiological properties of a protein inducing protoplast release during conjugation in theClosterium peracerosum-strigosum-littorale complex. Planta182: 348–354.CrossRefGoogle Scholar
  12. Sekimoto, H., Sone, Y. andFujii, T. 1994a. Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in theClosterium peracerosum-strigosum-littorale complex. Planta193: 137–144.PubMedCrossRefGoogle Scholar
  13. Sekimoto, H., Sone, Y. andFujii, T. 1994b. cDNA cloning of a 42-Kilodalton subunit of protoplast-release-inducing protein fromClosterium. Plant Physiol.104: 1095–1096.PubMedCrossRefGoogle Scholar
  14. Sekimoto, H., Sone, Y. andFujii, T. 1994c. A cDNA encoding a 19-Kilodalton subunit of protoplast-release-inducing protein fromClosterium. Plant Physiol.105: 447.PubMedCrossRefGoogle Scholar
  15. Shure, M., Wessler, S. andFederoff, N. 1983. Molecular identification of theWaxy locus in maize. Cell35: 225–233.PubMedCrossRefGoogle Scholar
  16. Wendland, J., Vaillancourt, L.J., Hegner, J., Lengeler, K.B., Laddison, K.J., Specht, C.A., Raper, C.A andKothe, E. 1995. The mating-type locusBal ofSchizophyllum commune contains a pheromone receptor gene and putative pheromone genes. EMBO J.14: 5271–5278.PubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan 1997

Authors and Affiliations

  • Bunshiro Endo
    • 1
    • 2
    Email author
  • Tadashi Fujii
    • 3
  • Yuji Kamiya
    • 2
  • Hiroyuki Sekimoto
    • 4
  1. 1.Institute of Biological SciencesUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.Frontier Research Programthe Institute of Physical and Chemical Research (RIKEN)Wako-shi, SaitamaJapan
  3. 3.Department of Life Sciences, Faculty of Life SciencesToyo UniversityOura-gunJapan
  4. 4.Department of Life Sciences, Graduate School of Arts and SciencesUniversity of Tokyo, KOMABATokyoJapan

Personalised recommendations