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Archives of Dermatological Research

, Volume 288, Issue 12, pp 765–770 | Cite as

Stratum corneum lipids: the effect of ageing and the seasons

  • J. Rogers
  • C. Harding
  • A. Mayo
  • J. Banks
  • A. Rawlings
Original Paper

Abstract

Stratum corneum lipids play a predominant role in maintaining the water barrier of the skin. In order to understand the biological variation in the levels and composition of ceramides, ceramide 1 subtypes, cholesterol and fatty acids, stratum corneum lipids collected from tape strippings from three body sites (face, hand, leg) of female Caucasians of different age groups were analysed. In addition, we studied the influence of seasonal variation on the lipid composition of stratum corneum from the same body sites. The main lipid species were quantified using high-performance thin-layer chromatography and individual fatty acids using gas chromatography. Our findings demonstrated significantly decreased levels of all major lipid species, in particular ceramides, with increasing age. Similarly, the stratum corneum lipid levels of all the body sites examined were dramatically depleted in winter compared with spring and summer. The relative levels of ceramide 1 linoleate were also depleted in winter and in aged skin whereas ceramide 1 oleate levels increased. The other fatty acid levels remained fairly constant with both season and age, apart from lignoceric and heptadecanoic acid which showed a decrease in winter compared with summer. The decrease in the mass levels of intercellular lipids and the altered ratios of fatty acids esterified to ceramide 1, are likely to contribute to the increased susceptibility of aged skin to perturbation of barrier function and xerosis, particularly during the winter months.

Key words

Stratum corneum lipids Ceramides Ageing Seasons Ceramide 1 linoleate 

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References

  1. 1.
    Abe T, Mayuzumi J, Kikuchi N, Arai S (1989) Seasonal variations in skin temperature, skin pH, evaporative water loss and skin surface lipid values on human skin. Chem Pharm Bull 28: 387–392Google Scholar
  2. 2.
    Abraham W, Wertz P, Downing D (1985) Linoleate-rich acyl-glucosylceramides of pig epidermis: structure determination by proton magnetic resonance. J Lipid Res 26: 761–766PubMedGoogle Scholar
  3. 3.
    Akimoto K, Yoshikawa N, Higaki Y, Kawashima M, Imokawa G (1993) Quantitative analysis of stratum corneum lipids in xerosis and asteatotic eczema. J Dermatol 20: 1–6PubMedGoogle Scholar
  4. 4.
    Bowser P, White R (1985) Isolation barrier properties and lipid analysis of the stratum compactum—a discrete region of the stratum corneum. J Invest Dermatol 112: 1–14Google Scholar
  5. 5.
    Brincat M, Moniz CF, Studd JWW, Darby AJ, Magos A, Cooper D (1983) Sex hormones and skin collagen content in postmenopausal women. BMJ 287: 1337–1338PubMedCrossRefGoogle Scholar
  6. 6.
    Brod J, Traitler H, Studer A, de la Charrier V (1988) Evolution of lipid composition in skin treated with blackcurrant seed oil. Int J Cosmet Sci 10: 149–159PubMedGoogle Scholar
  7. 7.
    Burr G, Burr M (1929) A new deficiency disease produced by rigid exclusion of fat from the diet. J Biol Chem 82: 345–367Google Scholar
  8. 8.
    Denda M, Koyama J, Hori J, Horii I, Takahashi M, Hara M, Tagami H (1993) Age- and sex-dependent change in stratum corneum sphingolipids. Arch Dermatol Res 285: 415–417PubMedCrossRefGoogle Scholar
  9. 9.
    Denda M, Koyama R, Horii I (1994) Stratum corneum lipid morphology and transepidermal water loss in normal skin and surfactant-induced scaly skin. Arch Dermatol Res 286: 41–46PubMedCrossRefGoogle Scholar
  10. 10.
    Elias P, Friend D (1975) The permeability barrier in mammalian epidermis. J Cell Biol 65: 180–191PubMedCrossRefGoogle Scholar
  11. 11.
    Elias P, Menon G (1991) Structural and lipid biochemical correlates’ of the epidermal permeability barrier. In: Elias P (ed) Skin lipid. Advances in lipid research, Academic Press, San Diego 24, p 26Google Scholar
  12. 12.
    Elias P, Williams M, Maloney M, Bonifas J, Brown B, Grayson S, Ervin H, Epstein J (1984) Stratum corneum lipids in disorders of cornification. J Clin Invest 74: 1414–1421PubMedGoogle Scholar
  13. 13.
    Ghadially R, Brown B, Sequeira-Martin S, Feingold K, Elias P (1995) The aged epidermal permeability barrier. J Clin Invest 95: 2281–2290PubMedCrossRefGoogle Scholar
  14. 14.
    Gluden G, Guzek D, Kennedy A, Mckie J, Potts R (1989) Stratum corneum phase transitions and water barrier properties. Biochemistry 26: 2382–2388CrossRefGoogle Scholar
  15. 15.
    Grove GL, Kligman AM (1983) Age-associated change in human epidermal cell renewal. J Gerontol 38: 137–142PubMedGoogle Scholar
  16. 16.
    Herrmann S, Scheuber E, Plewig G (1983) Exfoliative cytology: effects of seasons. In: Marks R (ed) The stratum corneum. Springer, Berlin Heidelberg New York, pp 181–185Google Scholar
  17. 17.
    Hou S, White S, Menon G, Grayson S, Ghadially R, Elias P (1991) Membrane structures in normal and essential fatty acid deficient stratum corneum: characteristics by ruthenium tetroxide staining and x-ray diffraction. J Invest Dermatol 96: 215–223PubMedCrossRefGoogle Scholar
  18. 18.
    Imokawa G, Hattori M (1985) A possible function of structural lipid in the water holding properties of the stratum corneum. J Invest Dermatol 84: 282–284PubMedCrossRefGoogle Scholar
  19. 19.
    Imokawa G, Akasaki S, Hattori M, Yoshizuka N (1986) Selective recovery of deranged water-holding properties by stratum corneum lipids. J Invest Dermatol 87: 758–761PubMedCrossRefGoogle Scholar
  20. 20.
    Imokawa G, Abe A, Jin K, Higaki Y, Kawashima M, Hidano A (1991) Decreased levels of ceramides in stratum corneum of atopic dermatitis: an etiologic factor in atopic dry skin. J Invest Dermatol 96: 523–526PubMedCrossRefGoogle Scholar
  21. 21.
    Lundstrom A, Egulrud T (1989) Cell shedding from human plantar skin in vitro: evidence that two different types of protein structures are degraded by a chymotrypsin-like enzyme. Arch Dermatol Res 282: 234–237CrossRefGoogle Scholar
  22. 22.
    Malkinson FD (1958) Studies of the percutaneous absorption of C14 labelled steroids by use of the gas-flow cell. J Invest Dermatol 31: 19–28PubMedCrossRefGoogle Scholar
  23. 23.
    Man M, Feingold K, Elias P (1993) Exogenous lipids influence permeability barrier recovery in acetone-treated murine skin. Arch Dermatol 129: 728–738PubMedCrossRefGoogle Scholar
  24. 24.
    Marks R, Lawson A, Nicholls S (1983) Age-related changes in stratum corneum, structure and function. In: Marks R (ed) The stratum corneum. Springer, Berlin Heidelberg New York, pp 175–180Google Scholar
  25. 25.
    Niemenen E, Leikola E, Koijoner M, Kiistala U, Mestakillo K (1967) Quantitative analysis of epidermal lipids by thin layer chromatography with specific reference to seasonal and age variations. Acta Derm Venereol 47: 327–338Google Scholar
  26. 26.
    Oldroyd J, Critchley P, Tiddy G, Turner J, Rawlings A (1994) Specialised role for ceramide one in the stratum corneum water barrier. J Invest Dermatol 102: 525Google Scholar
  27. 27.
    Potts RO, Francoeur ML (1991) The influence of stratum corneum morphology on water permeability. J Invest Dermatol 96: 495–499PubMedCrossRefGoogle Scholar
  28. 28.
    Rawlings A, Critchley P, Ackerman C, Parnell A, Rogers J, Oldroyd J (1992) The functional roles of ceramide one. Proceed 17th IFSCC, International federation societies of cosmetic chemists, Vienna. Int Congress 1: 14Google Scholar
  29. 29.
    Rawlings A, Watkinson A, Harding C, Ackerman C, Banks J, Hope J, Scott I (1992) Changes in stratum corneum lipid structure and water barrier function during mechanical stress. Proceed 17th IFSCC, International federation societies of cosmetic chemists, Vienna. Int Congress 1: 14Google Scholar
  30. 30.
    Rawlings A, Hope J, Watkinson A, Harding C, Egelrud T (1993) The biological effect of glycerol. J Invest Dermatol 100: 526Google Scholar
  31. 31.
    Rawlings A, Hope J, Rogers J, Mayo A, Watkinson A, Scott I (1994) Abnormalities in sratum corneum structure, lipid composition and desmosome degradation in soap induced winter xerosis. J Soc Cosmet Chem 45: 203–220Google Scholar
  32. 32.
    Rawlings A, Bowser P, Harding C, Scott I (1994) Stratum corneum moisturisation at the molecular level. J Invest Dermatol 103: 1–10CrossRefGoogle Scholar
  33. 33.
    Roskos K, Guy R (1989) Assessment of skin barrier function using transepidermal water loss: effect of age. Pharm Res 6: 949–953PubMedCrossRefGoogle Scholar
  34. 34.
    Saint-Leger D, Francois A, Leveque J, Stoudemayer T, Kligman A, Grove G (1989) Stratum corneum lipids in xerosis. Dermatologica 178: 151–155PubMedGoogle Scholar
  35. 35.
    Scott I, Harding C (1986) Fillagrin breakdown to water binding compounds during development of the rat stratum corneum is controlled by the water activity of the environment. Dev Biol 115: 84PubMedCrossRefGoogle Scholar
  36. 36.
    Shuster S, Black MM, McVitie E (1975) The influence of age and sex on skin thickness, skin collagen and density. Br J Dermatol 93: 639–643PubMedCrossRefGoogle Scholar
  37. 37.
    Sugino K, Imokawa G, Maibach H (1993) Ethnic differences of stratum corneum lipid in relation to stratum corneum function. J Invest Dermatol 100: 587Google Scholar
  38. 38.
    Urano R, Sakabe K, Kawashima I, Ohkido M, Seiki K (1992) Effects of estrogen and progesterone on induction and proliferation of normal human keratinocytes. J Dermatol Sci 4: 141CrossRefGoogle Scholar
  39. 39.
    Watkinson A, Smith G, Rawlings A (1994) Identification and localization of tryptic and chymotryptic like enzymes in human stratum corneum. J Invest Dermatol 102: 637Google Scholar
  40. 40.
    Wertz P, Downing (1983) Acylglucoceramides of pig epidermis; structure determination. J Lipid Res 24: 753–758PubMedGoogle Scholar
  41. 41.
    Wertz P, Downing D (1983) Ceramides of pig epidermis: structure determination. J Lipid Res 24: 759–765PubMedGoogle Scholar
  42. 42.
    Wertz PW, miethke MC, Long SA, Strauss JS, Downing DT (1985) The composition of the ceramides from human stratum corneum and from comedones. J Invest Dermatol 84: 410–412PubMedCrossRefGoogle Scholar
  43. 43.
    Yoshikawa N, Imokawa G, Akimoto K, Jin K, Higaki Y, Kawashima M (1994) Regional analysis of ceramides within the stratum corneum in relation to seasonal changes. Dermatology 188: 207–214PubMedCrossRefGoogle Scholar
  44. 44.
    Yumamoto A, Serizawa M, Ito M, Sato Y (1991) Stratum corneum lipid abnormalities in atopic dermatitis. Arch Dermatol Res 283: 219–223CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. Rogers
    • 1
  • C. Harding
    • 1
  • A. Mayo
    • 1
  • J. Banks
    • 1
  • A. Rawlings
    • 2
  1. 1.Unilever ResearchColworth LaboratorySharnbrookUK
  2. 2.Unilever ResearchEdgewaterUSA

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