Advertisement

Plant Molecular Biology

, Volume 4, Issue 1, pp 13–22 | Cite as

The presence and photoregulation of protochlorophyllide reductase in green tissues

  • W. Trevor Griffiths
  • Steve A. Kay
  • Richard P. Oliver
Article

Summary

The enzyme protochlorophyllide (pchlide) reductase has been identified amongst the peptides, resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), of chloroplast membranes from oat and barley plants. In support of this identification the enzymic activity associated with the enzyme has also been measured in the same preparations. A higher level of enzyme was found in plants which had been darkened prior to extraction. Based on this data, mechanisms for the light regulated diurnal variation of the reductase are discussed.

Keywords

protochlorophyllide reductase regulation greening chloroplasts 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Apel K: The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Phytochrome-induced decrease of translatable mRNA coding for the NADPH-protochlorophyllide oxido-reductase. Eur J Biochem 120:89–93, 1981.PubMedCrossRefGoogle Scholar
  2. 2.
    Beale SI, Castelfranco PA:24C incorporation from exogenous compounds intoδ-aminolevulinic acid by greening cucumber cotyledons. Biochem Biophys Res Comm 52:143–149, 1973.PubMedCrossRefGoogle Scholar
  3. 3.
    Beer NS, Griffiths WT: Purification of the enzyme NADPH-protochlorophyllide oxidoreductase. Biochem J 195:83–92, 1981.PubMedGoogle Scholar
  4. 4.
    Bonner WM, Laskey RA: A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88, 1974.PubMedCrossRefGoogle Scholar
  5. 5.
    Bramhall S, Noack N, Wu M, Loewenberg JR: A simple colorimetric method for the determination of protein. Anal Biochem 31:146–148, 1969.PubMedCrossRefGoogle Scholar
  6. 6.
    Fraker PJ, Speek JC: Protein and cell membrane iodinations with a sparingly soluble chloramide 1,3,4,6,-tetrachloro-3a,6a-diphenyl-glycoluril. Biochem Biophys Res Comm 80:849–852, 1978.PubMedCrossRefGoogle Scholar
  7. 7.
    Griffiths WT: Reconstitution of chlorophyllide formation by isolated etioplast membranes. Biochem J 174:681–692, 1978.PubMedGoogle Scholar
  8. 8.
    Jones OTG, Saunders VA: Energy-linked transfer reactions inRhodopseudomonas viridis. Biochim Biophys Acta 275:427–436, 1972.PubMedCrossRefGoogle Scholar
  9. 9.
    Jones OTG: In: Dawson RMC, Elliot DC, Elliot WH, Jones KM (eds), Data for Biochemical Research. Clarendon Press, Oxford, pp 318–325, 1969.Google Scholar
  10. 10.
    Kay SA, Griffiths WT: Light induced breakdown of NADPH-Protochlorophyllide oxidoreductasein vitro. Plant Physiol 72:229–237, 1983.PubMedCrossRefGoogle Scholar
  11. 11.
    Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970.PubMedCrossRefGoogle Scholar
  12. 12.
    Mapelston RE, Griffiths WT: Effects of illumination of whole barley plants on the protochlorophyllide-activating system in the isolated plastids. Biochem Soc Trans 5:319–321, 1979.Google Scholar
  13. 13.
    Mapelston RE, Griffiths WT: Light-modulation of the activity of protochlorophyllide reductase. Biochem J 189:125–133, 1980.Google Scholar
  14. 14.
    Meyer G, Bliedung H, Kloppstech K: Protochlorophyllide oxidoreductase. Reciprocal regulation in mono- and dicotoledonous species. Plant Cell Reports 2:26–30, 1983.Google Scholar
  15. 15.
    Oliver RP, Griffiths WT: Covalent labelling of the NADPH:protochlorophyllide oxidoreductase from etioplast membranes with [3H]-N-phenylmaleimide. Biochem J 195:93–101, 1981.PubMedGoogle Scholar
  16. 16.
    Santel HJ, Apel K: The protochlorophyllide holochrome of barley (Hordeum vulgare L.). The effect of light on the NADPH:protochlorophyllide oxidoreductase. Eur J Biochem 120:95–103, 1981.PubMedCrossRefGoogle Scholar
  17. 17.
    Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Nat Acad Sci USA 76:4350–4354, 1979.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • W. Trevor Griffiths
    • 1
  • Steve A. Kay
    • 1
  • Richard P. Oliver
    • 2
    • 1
  1. 1.Department of BiochemistryUniversity of BristolBristolU.K.
  2. 2.Carlsberg Research LaboratoryValby, CopenhagenDenmark

Personalised recommendations