The botanical magazine = Shokubutsu-gaku-zasshi

, Volume 92, Issue 3, pp 163–171 | Cite as

Tissue culture inHaworthia

II. Effects of three auxins and kinetin on greening and redifferentiation of calluses
  • Yasunari Ogihara


Effects of three different auxins and kinetin in various combinations on greening and redifferentiation of the callus ofHaworthia setata were investigated. All auxins at the concentration of 50 mg/l inhibited callus greening. NAA in combination with kinetin promoted both callus greening and production of redifferentiated shoots. Low concentrations of IAA without kinetin promoted redifferentiation of shoots, but not callus greening. Addition of 2,4-D completely inhibited both greening and redifferentiation regardless of the level of kinetin except for the effects on shoot formation in the medium with 0.1 mg/l 2,4-D added. The calluses with the highest chlorophyll content were observed in the medium containing 2.0 mg/l kinetin without any auxins or with 0.1 mg/l NAA added. Most frequent shoot redifferentiation was observed in the medium containing 0.1 mg/l IAA without kinetin (redifferentiation rate; 67%), followed by the medium containing 10 mg/l NAA with 2.0 mg/l kinetin (44%), and 0.1 mg/l 2,4-D with 0.2 mg/l kinetin (33%). Generally, higher degrees of greening were associated with better growth. However, the auxins (IAA, NAA and 2,4-D) given at concentrations optimal for growth did not exhibit the highest degree of callus greening. Differences of the three auxins in their actions and interaction with kinetin were disclosed.


Chlorophyll Content Kinetin Shoot Formation Callus Growth Tobacco Callus 
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indole-3-acetic acid


1-naphthaleneacetic acid


2,4-dichlorophenoxyacetic acid


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Copyright information

© The Botanical Society of Japan 1979

Authors and Affiliations

  • Yasunari Ogihara
    • 1
  1. 1.Laboratory of Genetics, Faculty of AgricultureKyoto UniversityKyoto

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