, Volume 57, Issue 1–2, pp 29–35 | Cite as

Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

  • Y. Shi
  • Y. Sun
Originals Column Liquid Chromatography


A novel rigid spherical biporous poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene andn-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity and column efficiency at high flow rates.

Key Words

Column liquid chromatography Biporous matrix Radical suspension copolymerization Proteins 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • Y. Shi
    • 1
  • Y. Sun
    • 1
  1. 1.Department of Biochemical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinP.R. China

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