Advertisement

Chromatographia

, Volume 57, Issue 1–2, pp 29–35 | Cite as

Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

  • Y. Shi
  • Y. Sun
Originals Column Liquid Chromatography

Summary

A novel rigid spherical biporous poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene andn-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity and column efficiency at high flow rates.

Key Words

Column liquid chromatography Biporous matrix Radical suspension copolymerization Proteins 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Zeng, X.; Ruckenstein, E.J. Membrane Sci. 1998,148, 195–205.CrossRefGoogle Scholar
  2. [2]
    Garcia, M.C.; Marina, M.L.; Torre, M.J. Chromatogr. A 2000,880, 169–187.CrossRefGoogle Scholar
  3. [3]
    Horváth, J.; Boschetti, E.; Guerrier, L.; Cooke, N.J. Chromatogr. A 1994,679, 11–22.CrossRefGoogle Scholar
  4. [4]
    Tennikov, M.B.; Gazdina, N.V.; Tennikova, T.B.; Svec, F.J. Chromatogr. A 1998,798, 55–64.CrossRefGoogle Scholar
  5. [5]
    Afeyan, N.B.; Gordon, N.F.; Mazasaroff, I.; Varady, L.; Fulton, S.P.; Yang, Y.B.; Regnier, F.E.J. Chromatogr. 1990,519, 1–29.CrossRefGoogle Scholar
  6. [6]
    Carta, G.; Rodrigues, A.E.Chem. Eng. Sci. 1993,48, 3927–3935.CrossRefGoogle Scholar
  7. [7]
    Frey, D.; Schwesnheim, E.; Horváth, Cs.Biotechnol. Prog. 1993,9, 93–100.CrossRefGoogle Scholar
  8. [8]
    Zhang, M.L.; Sun, Y.J. Chromatogr. A 2001,922, 77–86.CrossRefGoogle Scholar
  9. [9]
    Shi, Y.; Dong, X.-Y.; Sun, Y.Chromatographia 2002,55(7/8), 405–410.CrossRefGoogle Scholar
  10. [10]
    Yu, Y.H.; Sun, Y.J. Chromatogr. A 1999,855, 129–136.CrossRefGoogle Scholar
  11. [11]
    Zhou, X.; Xue, B.; Sun, Y.Biotechnol. Prog. 2001,17, 1093–1098.CrossRefGoogle Scholar
  12. [12]
    Wu, G.; Brown, G.R.React. Polym. 1991,14, 49–61.CrossRefGoogle Scholar
  13. [13]
    He, L.Z.; Gan, Y.R.; Sun, Y.Bioprocess Eng. 1997,17(5), 301–305.CrossRefGoogle Scholar
  14. [14]
    Kegbubgerm, A.L.Principles of Biochemistry, Worth, New York,1982.Google Scholar
  15. [15]
    Guyot, A.; Bartholin, M.Prog. Polym. Sci. 1982,8, 277–331.CrossRefGoogle Scholar
  16. [16]
    Nash, D.C.; Chase, H.A.J. Chromatogr. A 1998,807, 185–207.CrossRefGoogle Scholar
  17. [17]
    Boyer, P.M.; Hsu, J.T.AIChE J. 1992,38, 259–272.CrossRefGoogle Scholar
  18. [18]
    Levison, P.R.; Mumford, C.; Streater, M.; Brandt-Nielsen, A.; Pathirana, N.D.; Badger, S.E.J. Chromatogr. A 1997,760, 151–158.CrossRefGoogle Scholar
  19. [19]
    Van Deemter, J.J.; Zuiderweg, F.J.; Klinkenberg, A.Chem. Eng. Sci. 1956,5, 271–289.CrossRefGoogle Scholar
  20. [20]
    Deen, W.M.AIChE J. 1987,33, 1409–1425.CrossRefGoogle Scholar
  21. [21]
    Nash, D.C.; Chase, H.A.J. Chromatogr. A 1997,776, 65–73.CrossRefGoogle Scholar
  22. [22]
    Staby, A.; Jensen, I.H.; Mollerup, I.J. Chromatogr. A 2000,897, 99–111.CrossRefGoogle Scholar
  23. [23]
    Gowanlock, D.; Bailey, R.; Cantwell, F.F.J. Chromatogr. A 1996,726, 1–23.CrossRefGoogle Scholar
  24. [24]
    Yang, Y.B.; Harrison, K.; Kindsvater, J.J. Chromatogr. A 1996,723, 1–10.CrossRefGoogle Scholar
  25. [25]
    Rendueles de la Vega, M.; Chenou, C.; Loureiro, J.M.; Rodrigues, A.E.Biochem. Eng. J. 1998,1, 11–23.CrossRefGoogle Scholar
  26. [26]
    Afeyan, N.B.; Fulton, S.P.; Regnier, F.E.J. Chromatogr. 1991,544, 267–279.CrossRefGoogle Scholar
  27. [27]
    Liapis, I.; McCoy, M.A.J. Chromatogr. 1992,599, 87–104.CrossRefGoogle Scholar
  28. [28]
    Rodrigues, A.E.J. Chromatogr. B 1997,699, 47–61.Google Scholar
  29. [29]
    Koh, J.-H.; Guiochon, G.J. Chromatogr. A 1998,796, 41–57.CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • Y. Shi
    • 1
  • Y. Sun
    • 1
  1. 1.Department of Biochemical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinP.R. China

Personalised recommendations