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Chromatographia

, Volume 55, Supplement 1, pp S65–S69 | Cite as

Towards molecular-imprint based SPE of local anaesthetics

  • L. I. Andersson
  • M. Abdel-Rehim
  • L. Nicklasson
  • L. Schweitz
  • S. Nilsson
Article

Summary

The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-analyte binding essentially unaffected. Efficient pre-concentration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.

Key Words

Solid-phase extraction Molecular imprinting Anaesthetics 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • L. I. Andersson
    • 1
  • M. Abdel-Rehim
    • 1
  • L. Nicklasson
    • 1
  • L. Schweitz
    • 2
  • S. Nilsson
    • 2
  1. 1.DMPK & Bioanalytical ChemistryAstraZeneca R&D SödertälieSödertäljeSweden
  2. 2.Technical Analytical ChemistryUniversity of LundLundSweden

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