Chromatographia

, Volume 54, Issue 3–4, pp 179–185

Analysis of vitamin E in food and phytopharmaceutical preparations by HPLC and HPLC-APCI-MS-MS

  • W. M. Stöggl
  • C. W. Huck
  • H. Scherz
  • M. Popp
  • G. K. Bonn
Originals

DOI: 10.1007/BF02492241

Cite this article as:
Stöggl, W.M., Huck, C.W., Scherz, H. et al. Chromatographia (2001) 54: 179. doi:10.1007/BF02492241

Summary

The analysis of α, β, γ, δ-tocopherols, trienols, α-tocopheryl acetate and nicotinate (vitamin E) in complex matrices was carried out using a new liquid chromatographic (HPLC) method giving better separation efficiency, selectivity and sensitivity than that described in the literature. The use of normal-phase (NP)-HPLC on silica gel with issoctane-diisopropylether-1,4-dioxane as optimized mobilepphase yielded higher resolution than conventional reversed-phase (RP)-HPLC using methanol mobile phase. Identification of peaks was by UV-absorbance at 295 nm, diode array, or fluorescence detection (λex = 295 nm,λex = 330 nm). The latter was found to be more selective and ten times more sensitive than UV-absorbance detection. A quadrupole, ion-trap mass spectrometer with an atmospheric-pressure ionization (APCl) interface was used to detect vitamin E constituents in the femtomole range. With collision-induced dissociation (CID) in the ion source, which gave characteristic fragmentation, the identity of the investigated compounds could be confirmed. Plots of peak area versus amount injected allowed quantitation of α, β, γ, δ-tocopherols and-trienols, α-tocopheryl acetate and nicotinate in real samples such as peanut, almond, spinach, spelt grain bran, latex and tablets. The method described offers fast identification and quantitation of vitamin E constituents of complex biological origin.

Key Words

Column liquid chromatography Atmospheric pressure chemical ionization—mass spectrometry Vitamin E Foodstuffs 

Copyright information

© Friedr. Viewg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • W. M. Stöggl
    • 1
  • C. W. Huck
    • 1
  • H. Scherz
    • 2
  • M. Popp
    • 3
  • G. K. Bonn
    • 1
  1. 1.Institute of Analytical Chemistry and RadiochemistryLeopold-Franzens UniversityInnsbruckAustria
  2. 2.Deutsche Forschungsanstalt für LebensmittelchemieGarchingGermany
  3. 3.Bionorica Arzneimittel GmbHNeumarkt/OberpfalzGermany

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