, 55:171 | Cite as

Determination of diquat herbicide by selective enrichment by column chromatography on imprinted polymer immobilised on silica gel

  • B. B. Prasad
  • S. Banerjee
Originals Column Liquid Chromatography


A silica gel-bound, imprinted polymer for selective recognition of the diquat herbicide: 5,6-dihydropyrazino [1,2,3,4-lmn]-1,10-phenanthrolinium dichloride, is reported. It was used in the solid-phase extraction procedure, following liquid chromatography-differential pulse, adsorptive-stripping voltammetric (LC-DPAdSV) technique. The operational conditions for 100% retention of the diquat herbicide on a chromatographic column (13.0×1.4 cm, adsorbent bed 1.0g) were realized at pH 6.9 and a flow rate of 5.0 mL min−1. The adsorbent used could be regenerated during elution with 5.0 mL MHCl within 20 min. The limit of detection was 0.65 nM (0.18 ppb) with an enrichment factor as high as 99-fold.

Key Words

Column liquid chromatography Molecularly imprinted polymers Selective enrichment Differential pulse, adsorptive-stripping voltammetry Diquat herbicide 


  1. [1]
    Pichon, V.; Chen, L.; Hennion, M.C.; Daniel, R.; Martel, A.; LeGoffic, F.; Abian, J.; Barcelo, D.Anal. Chem. 1995,67, 2451–2460.CrossRefGoogle Scholar
  2. [2]
    Ferrer, I.; Lanza, F.; Tolokan, A.; Horvath, V.; Sellergren, B.; Horvai, G.; Barcelo, D.Anal. Chem. 2000,72, 3934–3941.CrossRefGoogle Scholar
  3. [3]
    Bjarnason, B.; Chimuka, L.; Ramstrom, O.Anal. Chem. 1999,71, 2152–2156.CrossRefGoogle Scholar
  4. [4]
    Kaiser, G.G.; Anderson, J.T.Fresenius J. Anal. Chem. 1992,342 (10), 834–838.CrossRefGoogle Scholar
  5. [5]
    Knutsson, M.; Nilve, G.; Mathiasson, L.; Jonsson, J.A.J. Chromatographia A 1996,754, 197–205.CrossRefGoogle Scholar
  6. [6]
    Lawrence, J.F.; Menard, C.; Hennion, M.C.; Pichon, V.; LeGoffic, F.; Durand, N.J. Chromatographia A 1996,752, 147–154.CrossRefGoogle Scholar
  7. [7]
    Pichon, V.; Chen, L.; Durand, N.; LeGoffic, F.; Hennion, M.C.J. Chromatogr. A,1996,725, 107–119.CrossRefGoogle Scholar
  8. [8]
    Land, C.C.J.LC-GC Int. 1994,7, 215–218.Google Scholar
  9. [9]
    (a) Wulff G. In polymeric Reagents and Catalysts, Ford, W.T. Ed.,A C S Symposium Series 308, American Chemical Society: Washington, DC,1986. (b) Wulff, G.; Vespor, W.; Grobo-Einsier, R.; Sarhan, A.Makromol. Chem. 1977,178, 2799. (c) Shea, K.J.; Dougherty, T.K.J. Am. Chem. Soc. 1986,108, 1091. (d) Wulff, G.; Heide, B.; Helfmeier, G.J. Am. Chem. Soc. 1986,108, 1089. (e) Andersson, L.; Sellergren, B.; Mosbach, K.Tetrahedron Lett. 1984,25, 5211.Google Scholar
  10. [10](a)
    Sellergren, B.; Lepisto, M.; Mosabach, K.J. Am. Chem. Soc. 1988,110, 5853, (b) Lepisto, M.; Sellergren, B.J. Org. Chem. 1989,54, 6010. (c) Sellergren, B.Chirality 1989,1, 63. (d) Andersson, L.I.; Mosbach, K.J. Chromatogr. 1990,516, 313. (e) Fischer, L.; Miller, R.; Ekberg, B.; Andersson, L.I.; Mosbach, K.J. Am. Chem. Soc. 1991,113, 9358–9360.CrossRefGoogle Scholar
  11. [11]
    Sellergren, B.; Shea, K.J.J. Chromatogr. 1993,635, 31–49.CrossRefGoogle Scholar
  12. [12]
    Shea, K.J.; Spivak, D.A.; Sellergren, B.J. Am. Chem. Soc. 1993,115, 3368–3369.CrossRefGoogle Scholar
  13. [13]
    Vlatakis, G.; Andersson, L.I.; Miller, R.; Mosbach, K.Nature 1993,361, 645–647.CrossRefGoogle Scholar
  14. [14]
    Wulff, G.ACS Symp. Ser. 1986, No.308, 186–230.CrossRefGoogle Scholar
  15. [15]
    Shea, K.J.Trends Polym. Sci. 1994,2, 166–173.Google Scholar
  16. [16]
    Mosbach, K.Trends Biochem. Sci. 1994,19, 9–14.CrossRefGoogle Scholar
  17. [17]
    Remcho, V.T.; Jessica, Z.Tan. Anal. Chem. News and Features,1999, 248–254.Google Scholar
  18. [18]
    Chen, Y.; Kele, M.; Saponz, P.; Sellergren, B.; Guichon, G.Anal. Chem. 1999,71, 928.CrossRefGoogle Scholar
  19. [19]
    Vidyasankar, S.; Ru, M.; Arnol, F.H.J. Chromatogr., A,1997,775, 51.CrossRefGoogle Scholar
  20. [20]
    Prasad, B.B.; Sundd, S.Bull. Chem. Soc. Jpn.,1995,68, 1–7.CrossRefGoogle Scholar
  21. [21]
    Bruggermann, O.; Freitag, R.; Whitcombe, M.J.; Vufson, E.N.J. Chromatogr., A,1997,781, 43–53.CrossRefGoogle Scholar
  22. [22]
    Dickeson, J.E.; Summers, L.A.J. Sci. Fd. Agric. 1969,20, 74.Google Scholar
  23. [23]
    Bellamy, C.J. “Advances in Infrared Frequencies”, Chaucer Press, Great Britain1968, p. 279.Google Scholar
  24. [24]
    Rochester, C.H.; Yong, G.H.J. Chem. Soc., Faraday Trans. 1 1980,76, 2142.CrossRefGoogle Scholar
  25. [25]
    Okada, T.Anal. Chem. 1998,70, 1692–1700.CrossRefGoogle Scholar
  26. [26]
    Kriz, D.; Ramström, O.; Mosbach, K.Anal. Chem.,1997,69, 345A.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • B. B. Prasad
    • 1
  • S. Banerjee
    • 1
  1. 1.Analytical Division, Chemistry Department, Faculty of ScienceBanaras Hindu UniversityVaranasiIndia

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