, Volume 57, Issue 9–10, pp 569–575 | Cite as

Use of monolithic supports for fast analysis of drugs and metabolites in plasma by direct injection

  • S. Souverain
  • S. Rudaz
  • J. -L. Veuthey
Originals Column Liquid Chromatography


Commercial monolithic columns were evcluated as extraction supports for the analysis of various drugs and their metabolites from plasma. For this purpose, a precolumn packed with a monolithic phase was used as extraction and enrichment support and integrated in a column-switching system for the direct and simultaneous analysis of fluoxetine and norfluoxetine, methadone and EDDP, flunitrazepam and norflunitrazepom in humon plasma. In this configuration, plasma samples were directly injected onto a Chromolith Flash precolumn (25×4.6 mm I.D.) coupled to a microbore analytical column packed with a conventional reversed-phase malerial. Detection was by an electrospray-ionization mass spectrometer (ESl-MS). Total analysis time, including extraction and separation of the six analytes, was <10 min. For all the investigated compounds, the limits of quantitation (LOQ) were estimated in the ng.mL1 range for an injection volume of 50 μL. The method was also applied to a real clinical case to demonstrate its applicability.

Key Words

Column liquid chromatography Monolithic columns Drugs and metabolites Fast analysis 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • S. Souverain
    • 1
  • S. Rudaz
    • 1
  • J. -L. Veuthey
    • 1
  1. 1.Laboratory of Analytical Pharmaceutical Chemistry, School of PharmacyUniversity of GenevaGeneva 4Switzerland

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