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Chromatographia

, Volume 57, Issue 3–4, pp 133–138 | Cite as

An on-line solid phase extraction—Liquid chromatography—Tandem mass spectrometry method for the analysis of citalopram, fluvoxamine, and paroxetine in human plasma

  • M. Kollroser
  • C. Schober
Originals Column Liquid Chromatography

Summary

A specific and sensitive direct-injection high performance liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (HPLC-APCI-MS-MS) method has been developed for the rapid identification and quantitative determination of citalopram, fluvoxamine, and paroxetine in human plasma. After dilution with 0.1% formic acid, plasma samples were injected into the LC-MS-MS system. Proteins and other large biomolecules were removed during an on-line sample cleanup step. The inter- and intra-assay coefficients of variation for all compounds were <11%. The total analysis time was 6 min per sample. The proposed method permits direct analysis of plasma samples without time-consuming sample preparation.

Key Words

Column liquid chromatography MS-MS detection On-line sample clean-up Citalopram, fluvoxamine and paroxetine Human plasma 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • M. Kollroser
    • 1
  • C. Schober
    • 2
  1. 1.Institute of Forensic MedicineKarl-Franzens University GrazGrazAustria
  2. 2.Institute of Molecular Biology, Biochemistry, and Microbiology (IMBM)Karl-Franzens University GrazGrazAustria

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