Chromatographia

, Volume 53, Issue 9–10, pp 574–578 | Cite as

HPLC validation of ameziniummetilsulfate in human plasma from uremia patient treated by dialysis

  • H. Shintani
  • F. Hayashi
Short Communications Column Liquid Chromatography
Received:
Revised:
Accepted:
  • 27 Downloads

Summary

A high-performance liquid chromatography (HPLC) method using an internal standard is validated for the determination of 4-amino-6-methoxy-1-phenyl-pyridazinium methyl sulfate (ameziniummetilsulfate, AM) in a uremia patient treated by artificial dialysis. The method involves liquid-liquid extraction and ion-supression, reverse-phase HPLC on an end-capped C-18 column. There is no interference by plasma components or AM metabolites in the HPLC analysis. Average recovery rate using liquid-liquid extraction was 88.7% and limit of determination was 2 ng mL−1. This concentration was sufficient to determine AM in human plasma. Accuracy and precision was <5.6% and 16.2%, respectively, at 2 ng mL−1, the limit of determination.

Key Words

Column liquid chromatography Ameziniummetilsulfate Uremia Dialysis 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • H. Shintani
    • 1
  • F. Hayashi
    • 2
  1. 1.National Institute of Health Sciences, Ministry & WelfareTokyoJapan
  2. 2.Health care facility of Namiki hospitalNagoyaJapan

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