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Chromatographia

, Volume 53, Issue 3–4, pp 131–134 | Cite as

Separation ofAstragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

  • M. Ganzera
  • E. Bedir
  • I. Calis
  • I. A. Khan
Originals Column Liquid Chromatography

Summary

A new HPLC method permitted the separation of 13 triterpene lycosides isolated from differentAstragalus species within 40 min. A water/acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins ofA. membranaceus could be detected at levels as low as 20.0 μg·mL−1. This method facilitated distinction of differentAstragalus species as well as the analyses of market products containingA. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

Key Words

Column liquid chromatography Evaporative light scattering detection Astragalus species Saponin 

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References

  1. [1]
    Calis, I.; Sticher, O.Triterpene Saponins from Plants of the Flora of Turkey, inSaponins Used in Traditional and Modern Medicine, Plenum Press, New York,1996.Google Scholar
  2. [2]
    Leung, A.; Foster, S.Encyclopedia of Common Natural Ingredients Used in Food, Drugs and Cosmetics, John Wiley & Sons New York,1996.Google Scholar
  3. [3]
    Foster, S.; Yue, C.X.Herbal Emissaries: Bringing Chinese Herbs to the West, Healing Arts Press, Rochester,1992.Google Scholar
  4. [4]
    Rios, J.L.; Waterman, P.G.Phytother. Res. 1997,11, 411–418.CrossRefGoogle Scholar
  5. [5]
    Craker, L.E.; Simons, J.Herbs, Spices and Medicinal Plants, Food Products Press, New York-London-Sydney,1987.Google Scholar
  6. [6]
    Bedir, E.; Pugh, N.; Calis, I.; Pasco, D.; Khan, I.A.Biol. Pharm. Bull. 2000,23, 834–837.Google Scholar
  7. [7]
    Bedir, E.; Calis, I.; Aquino, R.; Piacente, S.; Pizza, C.J. Nat. Prod. 1998,61, 1469–1472.CrossRefGoogle Scholar
  8. [8]
    Calis, I.; Yusufoglu, H.; Zerbe, O.; Sticher, O.Phytochemistry 1999,50, 843–847.CrossRefGoogle Scholar
  9. [9]
    Bedir, E.; Calis, I.; Zerbe, O.; Sticher, O.J. Nat. Prod. 1998,61, 503–505.CrossRefGoogle Scholar
  10. [10]
    Bedir, E.; Calis, I.; Aquino, R.; Piacente, S.; Pizza, C.J. Nat. Prod. 1999,62, 563–568.CrossRefGoogle Scholar
  11. [11]
    Bedir, E.; Calis, I.; Aquino, R.; Piacente, S.; Pizza, C.Phytochemistry 1999,51, 1017–1020.CrossRefGoogle Scholar
  12. [12]
    Kitagawa, I.; Wang, K.H.; Yashikowa, M.Chem. Pharm. Bull. 1983,31, 716–722.Google Scholar
  13. [13]
    Zhao, L.; Zhu, D.; Yan, Y.Yaowu Fenxi Zazhi 1999,19, 403–406.Google Scholar
  14. [14]
    Zhou, C.; Lu, J.Zhongguo Zhongyao Zazhi 2000,25, 166–168.Google Scholar
  15. [15]
    Ganzera, M.; Bedir, E.; Khan, I.A.Chromatographia 2000,52, 301–304.CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • M. Ganzera
    • 1
  • E. Bedir
    • 1
    • 2
  • I. Calis
    • 2
  • I. A. Khan
    • 1
    • 3
  1. 1.National Center for Natural Products Research, Research Institute of Pharmaceutical SciencesThe University of MississippiUniversityUSA
  2. 2.Department of Pharmacognosy, Faculty of PharmacyHacettepe UniversityAnkaraTurkey
  3. 3.Department of Pharmacognosy, School of PharmacyThe University of MississippiUniversityUSA

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