, Volume 53, Issue 3–4, pp 131–134 | Cite as

Separation ofAstragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

  • M. Ganzera
  • E. Bedir
  • I. Calis
  • I. A. Khan
Originals Column Liquid Chromatography


A new HPLC method permitted the separation of 13 triterpene lycosides isolated from differentAstragalus species within 40 min. A water/acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins ofA. membranaceus could be detected at levels as low as 20.0 μg·mL−1. This method facilitated distinction of differentAstragalus species as well as the analyses of market products containingA. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

Key Words

Column liquid chromatography Evaporative light scattering detection Astragalus species Saponin 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • M. Ganzera
    • 1
  • E. Bedir
    • 1
    • 2
  • I. Calis
    • 2
  • I. A. Khan
    • 1
    • 3
  1. 1.National Center for Natural Products Research, Research Institute of Pharmaceutical SciencesThe University of MississippiUniversityUSA
  2. 2.Department of Pharmacognosy, Faculty of PharmacyHacettepe UniversityAnkaraTurkey
  3. 3.Department of Pharmacognosy, School of PharmacyThe University of MississippiUniversityUSA

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