Chromatographia

, Volume 52, Issue 3–4, pp 165–168 | Cite as

Retention behavior of basic compounds on alkylphosphonate-modified magnesia-zirconia composite stationary phase in RPHPLC

  • Yu-Qi Feng
  • Hong-Jing Fu
  • Qing-He Zhang
  • Shi-Lu Da
  • You-Jie Zhang
Originals Column Liquid Chromatography

Summary

The chromatographic properties of an alkylphosphonate-modified magnesia-zirconia composite stationary phase have been investigated by reversed-phase high-performance liquid chromatography with basic compounds as probes. The influence of organic modifier composition and mobile phase pH was studied. The new stationary phase, similar to a silica-based reversed-phase stationary phase, has hydrophobic properties, but greater pH stability. Use of the phase results in more symmetric peaks for basic compounds.

A possible mechanism of retention of basic solutes on the new stationary phase is discussed. The chromatographic behavior of the basic solutes depends mainly on hydrophobic interactions between the solutes and the hydrophobic moiety of the stationary phase. Brönsted acidic and basic sites on the surface of the new stationary phase play an important role in the retention of ionized solutes by ion-exchange interaction. Promising separations of some basic compounds have been achieved by use of methanolic TRIS buffer, pH 10.0, as the mobile phase.

Key Words

Column liquid chromatography Stationary phase Alkylphosphonate-modified magnesia-zirconia Basic compounds 

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References

  1. [1]
    Kirkland, J.J.; Glajch, J.L.; Farlee, R.D.Anal. Chem. 1989,61, 2.Google Scholar
  2. [2]
    Kurganov, A.; Tevlin, A.; Davankov, V.J. Chromatogr. 1983,261, 479.CrossRefGoogle Scholar
  3. [3]
    Kaneko, S.; Mitsuzawa, T.; Ohmori, S.; Nakamura, M.; Nobuhara, K.; Masatani, M.J. Chromatogr. A 1994,669, 1.CrossRefGoogle Scholar
  4. [4]
    Nawrocki, J.; Rigney, M.P.; McCormic, A.; Carr, P.W.J. Chromatogr. A 1993,657, 229.CrossRefGoogle Scholar
  5. [5]
    Kurganov, A.; Trüdinger, U.; Isaeva, T.; Unger, K.Chromatographia 1996,42, 217.CrossRefGoogle Scholar
  6. [6]
    Grün, M.; Kurganov, A.A.; Schacht, S.; Schüth, F.; Unger, K.K.J. Chromatogr. A 1996,740, 1.CrossRefGoogle Scholar
  7. [7]
    Trüdinger, U.; Müller, G.; Unger, K.K.J. Chromatogr. 1990,535, 111.CrossRefGoogle Scholar
  8. [8]
    Hanggi, D.A.; Marks, N.R.LC-GC 1993,11, 128.Google Scholar
  9. [9]
    Shargin, L.M.; Galkin, V.A.; Moiseev, V.E.; Ponomarieu, V.G.; Davankov, V.A.; Kurganov, A.A.; Ponomarieva, T.M.Zhur. Fiz. Khim. 1991,66, 2677.Google Scholar
  10. [10]
    Zhang, Q. -H.; Feng, Y. -Q.; Da, S. -L.Chromatographia 1999,50, 654.Google Scholar
  11. [11]
    Feng, Y. -Q.; Zhang, Q. -H.; Da, S. -L.; Zhang, Y.Chemical J. Chinese Univ. 1999,20 (Suppl.) 253.Google Scholar
  12. [12]
    Feng, Y. -Q.; Zhang, Q. -H.; Da, S. -L. Patent for Invention in China Appl. No. 9812696. X 1998.Google Scholar
  13. [13]
    Hu, H. -J.; Feng, Y. -Q.; Zhang, Q. -H.; Da, S. -L.Anal. Lett. 1999,32, 2761.Google Scholar
  14. [14]
    Feng, Y. -Q.; Zhang, Q. -H.; Da, S. -L.; Zhang, Y.Anal. Sci. in press.Google Scholar
  15. [15]
    Fu, H. -J.; Feng, Y. -Q.; Zhang, Q. -H.; Da, S. -L.; Zhang, Y. -J.Chinese J. Chromatogr. in press.Google Scholar
  16. [16]
    Ascah, T.L.; Feibush, B.J. Chromatogr. 1990,506, 357.CrossRefGoogle Scholar
  17. [17]
    Zhang, Q. -H.; Feng, Y. -Q.; Da, S. -L.Anal. Sci. 1999,15 767.CrossRefGoogle Scholar
  18. [18]
    Horváth, Cs.; Melander, W.; Molnar, J.Anal. Chem. 1977,49, 142.CrossRefGoogle Scholar
  19. [19]
    Leo, A.; Hansch, C.; Elkins, D.Chem. Rev. 1971, 71, 525.CrossRefGoogle Scholar
  20. [20]
    On-line log P calculation by Interactive LOGKOW Demo in http://esc.syrres. -com/ESC/≈-kowint.htmGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • Yu-Qi Feng
    • 1
  • Hong-Jing Fu
    • 2
  • Qing-He Zhang
    • 1
  • Shi-Lu Da
    • 1
  • You-Jie Zhang
    • 2
  1. 1.Department of ChemistryWuhan UniversityWuhanP.R. China
  2. 2.Center for Analysis and TestingCentral China Normal UniversityWuhanP.R. China

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