, Volume 56, Issue 1–2, pp 9–17 | Cite as

Laterally attached liquid-crystalline polymers as stationary phases. Effect of temperature in RP HPLC and comparison with a commercial C18 stationary phase

  • F. Girtti
  • G. Félix
  • M. -F. Achard
  • F. Hardouin
Originals Column Liquid Chromatography


Specific side-on-fixed liquid-crystalline polymers (SOLCP) have been synthesized for use in silica-modified stationary phases in high-performance liquid chromatography (HPLC). The mesogenic side group of the SOLCP is composed of three benzoate-type phenyl rings with terminal alkoxy chains and is laterally linked to a polysiloxane backbone via an alkyl ester spacer arm. The dependence of the logarithm of the retention factor on the reciprocal temperature showed that the liquid-crystalline anisotropic order was conserved in the small pores (200 Å diameter) of the silica gel. The first-order nematic-isotropic transition is lost and probably becomes second-order. Adsorption enthalpies for the liquid-crystalline stationary phases have been measurement for three polycyclic aromatic hydrocarbon isomers (ortho-terphenyl, triphenylene, and chrysene) and compared with those for a commercial C18 phase. The adsorption enthalpies never exceeded 30 kJ mol−1, i.e. ten times the thermal agitation energy,RT. They were always less on the SOLCP stationary phase than on the C18 column, emphasizing the more rigid structure of the liquid crystalline phase and its mechanism based upon adsorption. Better separation of steroids, pesticides and amino acids were obtained with the LCP-coated silica than the commercial bonded C18 column. Four small peptides were successfully separated by using pure water as mobile phase.

Key Words

Column liquid chromatography Liquid-crystalline polymers Shape recognition Local anisotropic order Polycyclic aromatic hydrocarbons 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • F. Girtti
    • 1
    • 2
  • G. Félix
    • 1
  • M. -F. Achard
    • 2
  • F. Hardouin
    • 2
  1. 1.E.N.S.C.P.B.Université Bordeaux IPessacFrance
  2. 2.C.R.P.P.-CNRSUniversité Bordeaux IPessacFrance

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