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Ultrastructural study of spore wall morphogenesis inOphioglossum thermale kom. var.nipponicum (Miyabe et Kudo) nishida

  • Koichi Uehara
  • Siro Kurita
Article

Abstract

Spore wall morphogenesis ofOphioglossum thermale var.nipponicum was examined by transmission electron microscopy. The spore wall of this species consists of three layers: endospore, exospore, and perispore. The spore wall development begins at the tetrad stage. At first, the outer undulating lamellar layer of the exospore (Lo) is formed on the spore plasma membrane in advance of the inner accumulating lamellar layer (Li) of the exospore. Next, the homogeneous layer of the exospore (H) is deposited on the outer lamellar layer. Both lamellar layers may be derived from spore cytoplasm; and the homogeneous layer, from the tapetum. Then the endospore (EN) is formed. It may be derived from spore cytoplasm. The membranous perispore (PE), derived from the tapetum, covers the exospore surface as the final layer. Though the ornamentation of this species differs distinctly from that ofO. vulgatum, the results mentioned above are fundamentally in accordance with the data obtained fromO. vulgatum (Lugardon, 1971). Therefore, the pattern of spore wall morphogenesis appears to be very stable in the genusOphioglossum.

Key words

exospore homogeneous layer lamellar layer Ophioglossum thermale var.nipponicum perispore spore wall 

Abbreviations

AC

archesporial cell

ap

aperture

d

dictyosome

dis.

distal face of the spore

ds

electron-dense substance

E

exospore

EN

endospore

er

endoplasmic reticulum

H

exospore homogeneous layer

L

exospore lamellar layer

Li

inner lamellar layer

li

lipid body

Lo

outer lamellar layer

Or

orbicule

p

plastid

PE

perispore

PL

plasmodium

pro.

proximal face of the spore

pt

peritapetal membrane

sc

surface coat

SMC

spore mother cell

SP

spore

SW

sporangium wall cell

T

tapetum

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Copyright information

© The Botanical Society of Japan 1989

Authors and Affiliations

  • Koichi Uehara
    • 1
  • Siro Kurita
    • 2
  1. 1.NODAI Research InstituteTokyo University of AgricultureTokyo
  2. 2.Department of Biology, Faculty of ScienceChiba UniversityChiba

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