Surgery Today

, Volume 28, Issue 7, pp 701–706 | Cite as

Detection of carcinoembryonic antigen mRNA in the mesenteric vein of patients with resectable colorectal cancer

  • Takashi Ueda
  • Junichiro Furui
  • Koh Komuta
  • Junzou Yamaguchi
  • Masayuki Yamamoto
  • Koichi Furukawa
  • Takashi Kanematsu
Original Articles


The detection of tumor cells in the drainage venous blood of patients with colorectal cancer was made feasible by demonstrating carcinoembryonic antigen (CEA) mRNA in the mononuclear cell component of the blood, using a nested reverse transcription-polymerase chain reaction. CEA mRNA was detected in the drainage venous blood from 11 (42%) of 26 patients, and the rate of detection increased according to the grade of vessel invasion. CEA mRNA was detected in all patients with synchronous liver metastases, even though there was no significant correlation between the presence of CEA mRNA in the drainage venous blood and the clinicopathological findings. As the presence of CEA mRNA in the drainage venous blood is an indicator of the spread of tumor cells in patients with colorectal cancer, this assay can be used to assess the possible outcome of patients with colorectal cancer, providing one more tool for the physician-oncologist to use in designing appropriate treatments.

Key Words

colorectal cancer CEA mRNA nested RT-PCR liver metastasis mesenteric vein 


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  1. 1.
    Greenway B (1988) Hepatic metastases from colorectal cancer: resection or not? Br J Surg 75:513–519CrossRefPubMedGoogle Scholar
  2. 2.
    Talbot IC, Ritchie S, Leighton MH, Hughes AO, Bussey HJR, Morson BC (1980) The clinical significance of invasion of veins by rectal cancer. Br J Surg 67:439–442CrossRefPubMedGoogle Scholar
  3. 3.
    Knudsen JB, Nilsson T, Sprechler M, Johansen A, Christensen N (1983) Venous and nerve invasion as prognostic factors in postoperative survival of patients with resectable cancer of the rectum. Dis Colon Rectum 26:613–617CrossRefPubMedGoogle Scholar
  4. 4.
    Jeng YW, Reiping T, Ming C (1994) Value of carcinoembryonic antigen in the management of colorectal cancer. Dis Colon Rectum 37:272–277CrossRefGoogle Scholar
  5. 5.
    Fisher ER, Turnbull RB (1955) The cytologic demonstration and significance of tumor cells in the mesenteric venous blood in patients with colorectal carcinoma. Surg Gynecol Obstet 100:102–108PubMedGoogle Scholar
  6. 6.
    Schlimok G, Funke I, Bock B (1990) Epithelial tumor cells in bone marrow of patients with colorectal cancer: immunocytochemical detection, phenotypic characterization, and prognostic significance. J Clin Oncol 8:831–837PubMedGoogle Scholar
  7. 7.
    Gerhard M, Juhl H, Kalthoff H, Schreiber HW, Wagener C, Neumaier M (1994) Specific detection of carcinoembryonic antigen expressing tumor cells in bone marrow aspirates by polymerase chain reaction. J Clin Oncol 12:725–729PubMedGoogle Scholar
  8. 8.
    Matsumura M, Niwa Y, Kato N (1994) Detection of α-fetoprotein mRNA, an indicator of hematogenous spreading hepatocellular carcinoma, in the circulation: a possible predictor of metastatic hepatocellular carcinoma. Hepatology 20:1418–1425CrossRefPubMedGoogle Scholar
  9. 9.
    Israeli RS, Miller WH, Su SL (1994) Sensitive nested reverse transcription polymerase chain reaction detection of circulating prostatic tumor cells: comparison of prostate-specific membrane antigen and prostate-specific antigen-based assays. Cancer Res 54:6306–6310PubMedGoogle Scholar
  10. 10.
    Fey MF, Kulozik AE, Hansen-Hagge TE, Tolber A (1991) The polymerase chain reaction: a new tool for the detection of minimal residual disease in hematological malignancies. Eur J Cancer 27:89–94CrossRefPubMedGoogle Scholar
  11. 11.
    Erlich HA, Gelfand D, Sninsky JJ (1991) Recent advances in the polymerase chain reaction. Science 252:1643–1651CrossRefPubMedGoogle Scholar
  12. 12.
    Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Ann Biochem 162:156–159CrossRefGoogle Scholar
  13. 13.
    Schrewe H, Thompson J, Bona M (1990) Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression. Mol Cell Biol 10:2738–2748PubMedGoogle Scholar
  14. 14.
    Yamashiro S, Ruan S, Furukawa K (1993) Genetic and enzymatic basis for the differential expression of GM2 and GD2 gangliosides in human cancer cell lines. Cancer Res 53:5395–5400PubMedGoogle Scholar
  15. 15.
    Hsu S, Raine L, Franger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques. J Histochem Cytochem 29:577–580PubMedGoogle Scholar
  16. 16.
    Kalthoff H, Schmiegel W, Roder C (1993) p53 and K-ras alterations in pancreatic epithelial lesion. Oncogene 8:289–298PubMedGoogle Scholar
  17. 17.
    Benhamou G, Marmuse JP, Johanet H, Goff JY (1990) Should liver metastasis from colorectal cancers be treated surgically? Lancet 335:482–3CrossRefPubMedGoogle Scholar
  18. 18.
    Kemeny N (1987) Role of chemotherapy in the treatment of colorectal carcinoma. Semin Surg Oncol 3:190–214CrossRefPubMedGoogle Scholar
  19. 19.
    Onodera H, Maetani S, Nishikawa T, Tobe T (1989) The reappraisal of prognostic classifications for colorectal cancer. Dis Colon Rectum 32:609–614CrossRefPubMedGoogle Scholar
  20. 20.
    Lindemann F, Schlimok G, Dirschedl P, Witte J, Riethmuller G (1992) Prognostic significance of micrometastatic tumor cells in bone marrow of colorectal cancer patients. Lancet 340:685–688CrossRefPubMedGoogle Scholar
  21. 21.
    Smith B, Selby P, Southgate J, Pittman K, Bradley C, Blair GE (1991) Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. Lancet 338:1227–1229CrossRefPubMedGoogle Scholar
  22. 22.
    Page M, Dalifard I, Daver A (1986) Immunostaining of colorectal cancer with monoclonal anti-CEA antibodies compared to serum and tumor CEA content. Anticancer Res 6:893–896PubMedGoogle Scholar
  23. 23.
    Audette M, Buchegger F, Schreyer M (1987) Monoclonal antibody against carcinoembryonic antigen (CEA) identifies two new forms of crossreacting antigens of molecular weight 90000 and 160000 in normal granulocytes. Mol Immunol 24:1177–1186CrossRefPubMedGoogle Scholar
  24. 24.
    Arakawa f, Kuroki M, Misumi Y (1990) Characterization of a cDNA clone encoding a new species of the nonspecific crossreacting antigen (NCA), a member of the CEA gene family. Biochem Biophys Res Commun 166:1063–1071CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Takashi Ueda
    • 1
  • Junichiro Furui
    • 1
  • Koh Komuta
    • 1
  • Junzou Yamaguchi
    • 1
  • Masayuki Yamamoto
    • 1
  • Koichi Furukawa
    • 2
  • Takashi Kanematsu
    • 1
  1. 1.Department of Surgery IINagasaki University School of MedicineNagasakiJapan
  2. 2.Department of OncologyNagasaki University School of MedicineNagasakiJapan

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