Expression of the mouse testicular histone gene H1t during spermatogenesis
- 106 Downloads
The testicular H1 histone variant, H1t, is synthesized during spermatogenesis in mammalian male germ cells. In situ hybridization and immunohistochemical techniques were used to assign the expression of either the H1t mRNA or the H1t protein to specific cell stages of spermatogenesis. Our results show the presence of the H1t mRNA only in the late and mid-pachytene stages, whereas the protein occurs first in pachytene spermatocytes, and persists until later stages from round up to elongated spermatids.
Unable to display preview. Download preview PDF.
- Gibson JS, Polak JM (1990) Principles and applications of complementary RNA probes. In Polak JM, McGee JOD (eds) In situ hybridization, principles and practice. Oxford University Press, Oxford, pp 81–94Google Scholar
- Russel LD, Ettlin RA, Sinha Hikim AP, Clegg ED (1990) Histological and histopathological evaluation of the testis. Cache River Press, Bolesta ClearwaterGoogle Scholar