Documenta Ophthalmologica

, Volume 74, Issue 3, pp 163–169 | Cite as

The mouse model of oxygen-induced retinopathy: A suitable animal model for angiogenesis research

  • Glen A. Gole
  • Jay Browning
  • Sonia M. Elts
Experimental Studies
Accepted:

Abstract

Newborn mice exposed to high (>98%) ambient oxygen during the newborn period and subsequently removed to room air will develop a proliferative retinopathy which mimics the neovascular component of acute retinopathy of prematurity. In this paper, we report preliminary ultrastructural findings on the vitreous new vessels in the mouse model of oxygen-induced retinopathy, and argue that the model is appropriate for research on non surgical treatments for ROP in particular and angiogenesis in general.

Key words

retinopathy of prematurity mouse oxygen-induced retinopathy angiogenesis ultrastructure 

Abbreviations

ROP

retinopathy of prematurity

mouse model

the mouse model of oxygen-induced retinopathy (described in full in text)

HRP

horseradish peroxidase

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References

  1. 1.
    Garner A. The pathology of retinopathy of prematurity. In: Silverman WA, Flynn JT, eds. Retinopathy of Prematurity. Boston: Blackwell, 1985: 19–52.Google Scholar
  2. 2.
    Gyllensten LJ, Hellstrom BE. Experimental approach to the pathogenesis of retrolental fibroplasia I. Changes to the eye induced by exposure of newborn mice to concentrated oxygen. Acta Pediat 1954; 43 (suppl): 131–48.Google Scholar
  3. 3.
    Gerschman R, Nadig PW, Snell AC, Nye SW. Effect of high oxygen concentrations on eyes of newborn mice. Am J Physiol 1954; 179: 115–8.PubMedGoogle Scholar
  4. 4.
    Ashton N, Ward B, Serpell G. Effect of oxygen on developing retinal vessels with particular reference to the problem of retrolental fibroplasia. Br J Ophthalmol 1954; 38: 397–432.PubMedGoogle Scholar
  5. 5.
    Ashton N. Donders lecture: Some aspects of the comparative pathology of oxygen toxicity in the retina. Br J Ophthalmol 1968; 52: 505–31.PubMedGoogle Scholar
  6. 6.
    Bischoff PM, Wajer SD, Flower RW. Scanning electron microscopic studies of the hyaloid vascular system in newborn mice exposed to O2 and CO2. Graefes Arch Klin Exp Ophthalmol 1983; 220: 257–63.Google Scholar
  7. 7.
    Patz A, Eastham A, Higgenbotham DH, Kleh T. Oxygen studies in retrolental fibroplasia II. The production of the microscopic changes of retrolental fibroplasia in experimental animals. Am J Ophthalmol 1953; 36: 1511–22.PubMedGoogle Scholar
  8. 8.
    Miller H, Miller B, Zonis S, Nir I. Diabetic neovascularization: permeability and ultrastructure. Invest Ophthalmol Vis Sci 1984; 25: 1338–1432.PubMedGoogle Scholar
  9. 9.
    Gole GA. MD thesis: Oxygen-Induced Retinopathy. Sydney: University of NSW, 1989.Google Scholar
  10. 10.
    Tasman W. Retinal detachment in retinopathy of prematurity. In: Silverman WA, Flynn JT, eds. Retinopathy of Prematurity. Boston: Blackwell, 1985: 229–38.Google Scholar
  11. 11.
    Cryotherapy for Retinopathy of Prematurity Study Group. Multicenter trial of cryotherapy for retinopathy of prematurity. Arch Ophthalmol 1988; 106: 471–9.Google Scholar
  12. 12.
    Folkman J. Successful treatment of an angiogenic disease (editorial). N Engl J Med 1989; 320: 1211–12.PubMedCrossRefGoogle Scholar
  13. 13.
    Gimbrone MA, Cotran RS, Leapman SB, Folkman J. Tumor growth and neovascularization: an experimental model using the rabbit cornea. JNCI 1974; 52: 413–27.PubMedGoogle Scholar
  14. 14.
    Adamson I, Jerdan JA, Lansing M, Martin GR, Glaser BM. Inhibition of neovascularization by a peptide analog of the cell-binding dominion of laminin. Invest Ophthalmol Vis Sci 1989; 30 (suppl): 391.Google Scholar
  15. 15.
    Phelps DL, Rosenbaum AL. Effects of marginal hypoxemia on recovery from oxygen induced retinopathy in the kitten model. Pediatrics 1984; 73: 1–6.PubMedGoogle Scholar
  16. 16.
    Patz A. Retrolental fibroplasia: experimental studies. Am J Ophthalmol 1955; 40: 174–83.PubMedGoogle Scholar
  17. 17.
    Ricci B, Calogero G. Oxygen-induced retinopathy in newborn rats: effects of prolonged normobaric and hyperbaric oxygen supplementation. Pediatrics 1988; 82: 193–8.PubMedGoogle Scholar
  18. 18.
    Gyllensten LJ, Hellstrom BE. Experimental approach to the pathogenesis of retrolental fibroplasia IV. The effects of gradual and rapid transfer from concentrated oxygen to normal air on the oxygen-induced changes in the eyes of young mice. Am J Ophthalmol 1956; 41: 619–27.PubMedGoogle Scholar
  19. 19.
    Patz A, Eastham AB. Oxygen studies in retrolental fibroplasia V. The effect of rapid vs. gradual withdrawal from oxygen on the mouse eye. Arch Ophthalmol 1957; 57: 724–9.Google Scholar
  20. 20.
    Gole GA, Belford DA, Rush RA. Laminin-like immunoreactivity in induced new vessels in kitten and mouse eyes. Aust NZ J Ophthalmol 1987; 15: 223–7.Google Scholar
  21. 21.
    Belford DA, Gole GA, Rush RA. Localization of laminin to retinal vessels of the rat and mouse using whole mounts. Invest Ophthalmol Vis Sci 1987; 28: 1761–6.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Glen A. Gole
    • 1
  • Jay Browning
    • 1
  • Sonia M. Elts
    • 1
  1. 1.Department of OphthalmologyThe Flinders University of South AustraliaAdelaideAustralia

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