Brain Tumor Pathology

, Volume 21, Issue 3, pp 105–112 | Cite as

Expression and quantitative analysis of matrix metalloproteinase-2 and-9 in human gliomas

  • Kyoko KomatsuEmail author
  • Yoko Nakanishi
  • Norimichi Nemoto
  • Tomokatsu Hori
  • Tatsuo Sawada
  • Makio Kobayashi
Original Article


The matrix metalloproteinase (MMP) family members catalyze extracellular proteolysis. Recent reports have suggested that expression of MMP-2 and-9 might play a critical role in neoplastic tissue invasion or metastasis. In this study, the relationship between the expression of MMP-2 and-9 and the histological features of tissues from 21 cases of human glioma were investigated. MMP-2 and-9 proteins were detected by immnohistochemical studies. Amplification of MMP-2 and-9 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) assay. MMP-2 and-9 mRNA was measured quantitatively by the real-time RT-PCR method. Immunohistochemically, 38% of the cases were positive for MMP-2. Amplification of MMP-2 mRNA by RT-PCR was detected in. 62% of the cases. There was no significant relationship between the expression of MMP-2 protein or mRNA and the biological nature of the tumors, including aggressiveness and histologic classification. The quantity of MMP-2 mRNA was 0.035±0.113 (MMP-2/GAPDH%), which was significantly elevated in cases of neoplastic dissemination or recurrence (P<0.05). Tumor cells were immunohistochemically positive for MMP-9 in 81% of the samples. A positive reaction was found not only in neoplastic cells but also in endothelial cells, suggesting that the expression of MMP-9 protein might be associated with tumoral angiogenesis. The expression of mRNA in MMP-9 was detected in 91% of the cases, suggesting a close relationship between expression of MMP-9 and malignancy. The quantity of MMP-9 was 0.097±0.113 (MMP-9/GAPDH %) in all samples, which was significantly elevated in cases of glioblastoma (P<0.05). The average Ki-67 labeling index was 8.14±5.26 in samples from G2 glioma, 19.92±11.29 in samples from G3 glioma, and 23.52±10.14 in samples from glioblastoma. All of the cases with elevated indices had recurrence or dissemination. The results of our study suggest that quantity analyses of MMP-2 and-9 mRNA and Ki-67 labeling index should be useful for discerning tumoral behaviors such as invasion, dissemination, and recurrence.

Key words

Glioma MMP-2 MMP-9 mRNA Quantity analysis 


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Copyright information

© The Japan Society of Brain Tumor Pathology 2004

Authors and Affiliations

  • Kyoko Komatsu
    • 1
    Email author
  • Yoko Nakanishi
    • 2
  • Norimichi Nemoto
    • 2
  • Tomokatsu Hori
    • 3
  • Tatsuo Sawada
    • 4
  • Makio Kobayashi
    • 4
  1. 1.Pathology LaboratoryNihon University Itabashi HospitalTokyoJapan
  2. 2.Department of PathologyNihon University School of MedicineTokyoJapan
  3. 3.Department of NeurosurgeryNeurological Institute, Tokyo Women's Medical UniversityTokyoJapan
  4. 4.Department of PathologyTokyo Women's Medical UniversityTokyoJapan

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