Brain Tumor Pathology

, Volume 21, Issue 1, pp 35–38

Expression of a transcriptional factor, SOX6, in human gliomas

  • Ryo Ueda
  • Kazunari Yoshida
  • Yutaka Kawakami
  • Takeshi Kawase
  • Masahiro Toda
Original Article

Abstract

By screening a human testis cDNA library with glioma patients' sera, we isolated a transcriptional factor, SOX6. Here, we analyzed SOX6 expression in gliomas having a range of malignancy grades using immunostaining. Murine Sox6 is a transcriptional factor that is specifically expressed in the developing central nervous system and in the early stages of chondrogenesis in mouse embryos. The reverse transcription-polymerase chain reaction (RT-PCR) revealed that the SOX6 gene was more highly expressed in glioma tissues and fetal brain than in normal adult brain and other cancer cells, except melanoma cells. Immunohistochemical analysis with the anti-SOX6 antibody showed that all the glioma tissues analyzed (14 glioblastomas, 14 anaplastic astrocytomas, 3 anaplastic oligoastrocytomas, 5 diffuse astrocytomas, 1 oligodendroglioma, and 1 pilocytic astrocytoma) expressed SOX6 in tumor cells, but only a few SOX6-positive cells were detected in nonneoplastic tissues from the cerebral cortex. These results indicate that the developmentally regulated transcription factor SOX6 may be a potential diagnostic marker for gliomas.

Key words

Glioma Transcriptional factor SOX6 

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References

  1. 1.
    Kurpad SN, Zhao XG, Wikstrand CJ, et al. (1995) Tumor antigens in astrocytic gliomas. Glia 15:244–256PubMedCrossRefGoogle Scholar
  2. 2.
    Ueda R, Iizuka Y, Yoshida K, et al (2004) Identification of a human glioma antigen, SOX6, recognized by patients' sera. Oncogene 23:1420–1427PubMedCrossRefGoogle Scholar
  3. 3.
    Kleihues P, Louis DN, Scheithauer BW, et al (2002) The WHO classification of tumors of the nervous system. J Neuropathol Exp Neurol 61:215–225PubMedGoogle Scholar
  4. 4.
    Yamashita A, Ito M, Takamatsu N, et al (2000) Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis. FEBS Lett 481:147–151PubMedCrossRefGoogle Scholar
  5. 5.
    Cohen-Barak O, Hagiwara N, Arlt MF, et al (2001) Cloning, characterization and chromosome mapping of the human SOX6 gene. Gene 265:157–164PubMedCrossRefGoogle Scholar
  6. 6.
    Laudet V, Stehelin D, Clevers H (1993) Ancestry and diversity of the HMG box superfamily. Nucleic Acids Res 21:2493–2501PubMedGoogle Scholar
  7. 7.
    Prior HM, Walter MA (1996) SOX genes: architects of development. Mol Med 2:405–412PubMedGoogle Scholar
  8. 8.
    Wegner M (1999) From head to toes: the multiple facets of Sox proteins. Nucleic Acids Res 27:1409–1420PubMedCrossRefGoogle Scholar
  9. 9.
    Connor F, Wright E, Denny P, et al (1995) The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse. Nucleic Acids Res 23:3365–3372PubMedGoogle Scholar
  10. 10.
    Lefebvre V, Li P, de Crombrugghe B (1998) A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene. Embo J 17:5718–5733PubMedCrossRefGoogle Scholar
  11. 11.
    Almqvist PM, Mah R, Lendahl U, et al (2002) Immunohistochemical detection of nestin in pediatric brain tumors. J Histochem Cytochem 50:147–158PubMedGoogle Scholar
  12. 12.
    Toda M, Iizuka Y, Yu W, et al (2001) Expression of the neural RNA-binding protein Musashil in human gliomas. Glia 34:1–7PubMedCrossRefGoogle Scholar

Copyright information

© The Japan Society of Brain Tumor Pathology 2004

Authors and Affiliations

  • Ryo Ueda
    • 1
  • Kazunari Yoshida
    • 2
  • Yutaka Kawakami
    • 3
  • Takeshi Kawase
    • 2
  • Masahiro Toda
    • 1
  1. 1.Neuroimmunology Research GroupKeio University School of MedicineTokyoJapan
  2. 2.Department of NeurosurgeryKeio University School of MedicineTokyoJapan
  3. 3.Division of Cellular Signaling, Institute for Advanced Medical ResearchKeio University School of MedicineTokyoJapan

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