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Brain Tumor Pathology

, Volume 15, Issue 1, pp 53–57 | Cite as

Function of aberrant EGFR in malignant gliomas

  • Noriaki Sugawa
  • Kazuaki Yamamoto
  • Satoshi Ueda
  • Noriyuki Morita
  • Masahide Kita
  • Hoyoku Nishino
  • Shinji Fushiki
  • Toshihide Okabe
Original Article

Abstract

The most common alteration of the epidermal growth factor receptor (EGFR) gene in human malignant gliomas is an in-frame deletion of exon 2–7 from the extracellular domain. To study the relationship between the expression of this aberrant EGFR and cell proliferation, as well as apoptosis in malignant gliomas, we have developed U-87MG cell transfectants that express the aberrant (mutant-type) or normal (wild-type) EGFR. We analyzed cell number, tumor volume, and MIB-1 positive rate as proliferation markers, and found that in tissue culture, tumors derived from U-87 MG cells (mutant-type) have the same proliferative activity as those derived from U-87 MG cells (wild-type). However, when cells expressing mutant EGFR were implanted into nude mice subcutaneously, the tumorigenic capacity was much enhanced. We also found that the apototic index of tumors derived from U-87 MG cells (mutant-type) was less than 0.1%, whereas that of wild-type tumor was 1%. These results suggest that aberrant EGFR affects the malignancy of glioma by stimulating proliferation and inhibiting apoptosis.

Key words

Glioma Aberrant EGFR Xenograftic tumor MB-1 Apoptosis 

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Copyright information

© The Japan Society of Brain Tumor Pathology 1998

Authors and Affiliations

  • Noriaki Sugawa
    • 1
  • Kazuaki Yamamoto
    • 2
  • Satoshi Ueda
    • 2
  • Noriyuki Morita
    • 3
  • Masahide Kita
    • 4
  • Hoyoku Nishino
    • 5
  • Shinji Fushiki
    • 6
  • Toshihide Okabe
    • 7
  1. 1.Department of NeurosurgeryKyoto Prefectural Yosanoumi HospitalKyotoJapan
  2. 2.Department of NeurosurgeryKyoto Prefectural University of MedicineKyotoJapan
  3. 3.Department of First AnatomyKyoto Prefectural University of MedicineKyotoJapan
  4. 4.Department of MicrobiologyKyoto Prefectural University of MedicineKyotoJapan
  5. 5.Department of BiochemistryKyoto Prefectural University of MedicineKyotoJapan
  6. 6.Research Institute for Neurogical Disease and GeviatricsKyotoJapan
  7. 7.Department of Clinical PathologyShiga Medical UniversityShigaJapan

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