Materials and Structures

, Volume 22, Issue 3, pp 163–169 | Cite as

Gas chromatographic detection of volatile amines found in indoor air due to putrefactive degradation of casein-containing building materials

  • S. Karlsson
  • Z. G. Bánhidi
  • A. -C. Albertsson
Article

Abstract

The use of non-sterile casein in building materials, especially in self-levelling floor-topping compound (putty), led to various defects in buildings with these materials. Bubbles in the mattings, dark patches unevenly spread over the floor and a very unpleasant odour developed. The defects were commonly induced or accelerated if the humidity in the floor was high. Our earlier studies have concluded that the cause of these problems is due to the growth of alkalitolerantClostridia. The presence of volatile mono-amines in air samples taken in the buildings also confirms our early theories. Due to the high pH of the putty, amines formed in the topping compound will easily be emitted into the air. Some of the amines are commonly found in spent growth media ofClostridia as well as in head-space samples from bacterial suspensions. By the use of gas chromatography (GC) we have been able to detect triethylamine, pyrrolidine, di-iso-butylamine, 2-ethyl-hexylamine and n-octylamine in the air samples. Air sampling was carried out by impinging in weak acidic solutions and adsorption on silica gel.

Along with the mono-amines, ammonia has also been detected. The sampling was carried out using Draeger Tubes, which also non-specifically indicate several volatile amines in addition to ammonia. The amount of amines recovered was well below official threshold limits. The amount was in the parts per billion (ppb) region, whereas official threshold limits are usually in the 1–25 parts per million (ppm) region.

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References

  1. 1.
    Albertsson, A.-C. and Bánhidi, Z. G., ‘Chemical and Bacteriological Studies in Connection with Floor-problems and Self-levelling Putty’ (Report to the Swedish Council for Building Research, 1983). Stockholm, Sweden.Google Scholar
  2. 2.
    Karlsson, S., Bánhidi, E., Bánhidi, Z. G. and Albertsson, A.-C., ‘Accumulation of malodorous amines and polyamines due to clostridial putrefaction indoors’. Proceedings of the 3rd International Conference on Indoor Air Quality and Climate Stockholm, 2:287–293.Google Scholar
  3. 3.
    Holdeman, L. V., Cato, E. P. and Moore, W. E. C., VPI Manual Anaerobic Laboratory Manual, 4th Ed., (Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA, 1977).Google Scholar
  4. 4.
    Karlsson, S., Bánhidi, Z. G. and Albertsson, A.-C., ‘Identification and characterization of alkali-tolerant Clostridia isolated from biodeteriorated casein containing building materials’,Appl. Microbiol. Biotechn.,27 (1988).Google Scholar
  5. 5.
    NIOSH, NIOSH Manual of Analytical Methods, USA, 2nd Ed. 1978, p. 4.Google Scholar
  6. 6.
    Mathiasson, L. and Lövkvist, P., ‘Comparison of column packings for the trace analysis of free amines by gas-liquid chromatography’,J. Chrom.,217 (1981) 177–181.CrossRefGoogle Scholar
  7. 7.
    Salle, A. J., ‘Fundamental Principles of Bacteriology’, 7th Ed. (Mc Graw-Hill 1973) p. 457.Google Scholar
  8. 8.
    Prevot, A. R. and Sarraff, A., ‘Recherches sur les odeurs dégagées par les bactéries anaérobies’, I–VII. Ann. Inst. Pasteur (1960–1962).Google Scholar
  9. 9.
    Larsson, L., Maardh, P.-A. and Odham, G., ‘Analysis of amines and other bacterial products by head-space gas chromatography’,Acta Path. Microbiol. Scand.,B 86 (1978) 207–213.Google Scholar
  10. 10.
    Audunsson, G. and Mathiasson, L., ‘Simultaneous determination of amines and isocyanates in working atmosphere by gas-liquid chromatography,’J. Chrom.,315 (1983) 253–264.CrossRefGoogle Scholar
  11. 11.
    Belin, L., Wass, U., Audunsson, G. and Mathiasson, L., ‘Amines: possible causative agents in the development of bronchial hyperreactivity in workers manufacturing polyurethanes from isocyanates’,Brit. J. Ind. Med.,40 (1983) 251–257.Google Scholar
  12. 12.
    Brubaker, R. E., Muranko, H. J., Smith, D. B., Beck, G. J. and Scovel, G., ‘Evaluation and control of a respiratory exposure to 3-(dimethylamino)-propylamine’,J. Occup. Med.,21 (1979) 688–690.Google Scholar
  13. 13.
    Hagmar, L., Bellander, T., Bergöö, B. and Simonsson, B. G., ‘Piperazine-induced occupational asthma’,J. Occup. Med.,24 (1982) 193–197.CrossRefGoogle Scholar

Copyright information

© RILEM 1989

Authors and Affiliations

  • S. Karlsson
    • 1
  • Z. G. Bánhidi
    • 1
  • A. -C. Albertsson
    • 1
  1. 1.Department of Polymer TechnologyThe Royal Institute of TechnologyStockholmSweden

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