Complement conversion and leukocyte kinetics in open heart surgery
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Complement conversions and a pulmonary leukocyte sequestration were observed during cardiopulmonary bypass (CPB) in all of twenty patients who received open heart surgery. A systemic neutropenia was initially observed in the early phase of CPB, and it subsequently turned into a systemic neutrocytosis during the late phase of it. A significant leukocyte sequestration was found in the pulmonary circulation during CPB coincidently with the systemic neutropenia. The contribution of the transpulmonary sequestration to the leukopenia was major, being as high as 80 per cent. Plasma C3, C4, and CH50 levels rapidly decreased following the commencement of CPB. Alterations of the C3 molecule in the patients’ plasma were demonstrated using the immunoblotting method. The appearances of C3a and C3b with the apparent molecular weights of 10,000 and 170,000 respectively, might be evidence of the complement activation during CPB. Another type of alteration in the C3 molecule was observed in the generation of a new fragment with the apparent molecular weight of 14,000. The appearance of this fragment, which did not share common epitopes with C3a, might suggest the consumption of complement components during CPB, unrelated to the activation of the complement systems.
Key Wordscardiopulmonary bypass leukocyte complement immunoblotting C3a
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- 3.Neville WE, Kentaxis A, Gavin T, Clowes GHA Jr. Post-perfusion pulmonary vasculitis. Its relation to blood trauma. Arch Surg 1963; 86: 126–137.Google Scholar
- 7.Caliunas P Jr, Chawla R, Lazarus JM, Cohn L, Sanders J, Merill JP. Acute renal failure following cardiac operations. J Thorac Cardiovasc Surg 1980; 79: 241–243.Google Scholar
- 16.Hugli TE, Cheneweth DE. Biologically active peptides of complement, techniques and significance of C3a and C5a measurements. In: Nakamura RM, Ditro WR, Tucher ES, eds. Immunoassays: clinical laboratory techniques for the 1980s. New York: Alan R Liss, 1980: 443–460.Google Scholar
- 18.Mayer MM. Complement and complement fixation. In: Kobat CA and Mayer MM, eds. Experimental immunochemistry. Springfield: Thomas, 1961: 133.Google Scholar
- 22.Guyton AC. a, Cardiac output, venous pressure, cardiac failure, and shock. b, Body fluids, capillary membrane dynamics, and the lymphatic system. c, Blood flow through the systemic circulation and its regulation. In: Guyton AC, ed. Physiology of the human body. 5th ed. Phyladelphia-London-Toronto: WB Saunders, 1979: 138–204.Google Scholar