, Volume 48, Issue 3–4, pp 197–202 | Cite as

Chiral recognition by molecularly imprinted polymers in aqueous media

  • O. Ramström
  • L. Ye
  • P. -E. Gustavsson


Molecularly imprinted polymers were prepared using 2-vinylpyridine and/or methacrylic acid as functional monomers in a self-assembly imprinting protocol. The resulting polymers were analyzed in aqueous media, and the effects from the pH of the mobile phase and the degree of added organic solvent were investigated. The results are indicative of the importance of ionic bonds in conjunction with hydrophobic interactions in the formation of the complexes between the analyte and the polymers.

Key Words

Column liquid chromatography Molecular imprinting Molecular recognition Chiral recognition 


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  1. [1]
    O. Ramström, R. J. Ansell, Chirality10, 195 (1998).CrossRefGoogle Scholar
  2. [2]
    K. Mosbach, O. Ramström, Bio/Technology,14, 163 (1996).CrossRefGoogle Scholar
  3. [3]
    S. Vidyasankar, F. H. Arnold, Curr. Opin. Biotechnol.6, 218 (1995).CrossRefGoogle Scholar
  4. [4]
    G. Wulff, Angew. Chem. Int. Ed. Engl.34, 1812 (1995).CrossRefGoogle Scholar
  5. [5]
    K. J. Shea, Trends Polym. Sci. (Cambridge, U. K.)2, 166 (1994).Google Scholar
  6. [6]
    M. J. Whitcombe, C. Alexander, E. N. Vulfson, Trends Food Sci. Technol.8, 140 (1997).CrossRefGoogle Scholar
  7. [7]
    L. I. Andersson, Anal. Chem.68, 111 (1996).CrossRefGoogle Scholar
  8. [8]
    L. I. Andersson, R. Muller, G. Vlatakis, K. Mosbach, Proc. Natl. Acad. Sci. U. S. A.92, 4788, (1995).CrossRefGoogle Scholar
  9. [9]
    K. Haupt, A. Dzgoev, K. Mosbach, Anal. Chem.70, 631 (1998).Google Scholar
  10. [10]
    C. Yu, O. Ramström, K. Mosbach, Anal. Lett.30, 2123 (1997).Google Scholar
  11. [11]
    I. Nicholls, O. Ramström, K. Mosbach, J. Chromatogr. A691, 349 (1995).CrossRefGoogle Scholar
  12. [12]
    S. Allenmark, Chromatographic Enantioseparation: Methods and Applications, Ellis Horwood Limited, Chichester, (1988).Google Scholar
  13. [13]
    L. R. Snyder, J. J. Kirkland, Introduction to modern liquid chromatography, Wiley Interscience Publishers, New York, (1974).Google Scholar
  14. [14]
    O. Ramström, L. I. Andersson, K. Mosbach, J. Org. Chem.58, 7562 (1993).CrossRefGoogle Scholar
  15. [15]
    J. March, Advanced Organic Chemistry, John Wiley & Sons, New York, ed. 4th Edition, (1992).Google Scholar
  16. [16]
    B. Sellergren, M. Lepistö, K. Mosbach, J. Am. Chem. Soc.110, 5853 (1988).CrossRefGoogle Scholar
  17. [17]
    B. Sellergren, K. J. Shea, J. Chromatogr. A654, 17 (1993).CrossRefGoogle Scholar
  18. [18]
    H.-J. Schneider, Angew. Chem. Int. Ed. Engl.30, 1417 (1991).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • O. Ramström
    • 1
  • L. Ye
    • 1
  • P. -E. Gustavsson
    • 1
  1. 1.Pure and Applied Biochemistry, Center for Chemistry and Chemical EngineeringLund UniversityLundSweden

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