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Chromatographia

, Volume 48, Issue 1–2, pp 120–126 | Cite as

Simultaneous GC-MS quantitation of o-phosphoric, aliphatic and aromatic carboxylic acids, proline, hydroxymethylfurfurol and sugars as their TMS derivatives: In honeys

  • K. Horváth
  • I. Molnár-Perl
Originals

Summary

A GC-MS procedure is described for the simultaneous quantitatation of the minor and major constituents of honeys, as their trimethylsilyl derivatives, from one solution, by one injection. Selected minor components (aliphatic and aromatic carboxylic acids, members of various homologous series, together with o-phosphoric acid, proline and hydroxymethylfurfurol), have been determined on the basis of their characteristic fragment ions, in the presence of extremely high excess of honeysaccharides. Selective fragmentation of these minor compounds in the ion trap detector provided possibilities for distinguishing them. The method permitted the simultaneous quantitation of o-phosphoric, malic, shikimic, citric/isocitric, quinic, margaric, oleic and stearic acids, hydroxymethylfurfurol and proline with the extremely high sugar contents of honeys (fructose, glucose, galacturonic acid, inositol, sucrose, trehalose, turanose, maltose, gentiobiose, isomaltose, raffinose, erlose, melezitose, maltotriose, panose, isomaltotriose) and allowed the fast evaluation of sugar and acid constituents of fifteen honeys from various floral and geological origin. Results revealed that (i) the minor components varied in the concentration range of 0.0001 to 0.43%, and, (ii) together with the saccharides of honeys made up the total of identified and determined constituents from 87.8% to 98.5%. Quantitative evaluation of the minor constituents was performed on the basis of their selective fragment ion values with an average reproducibility of 6.7% (RSD).

Key Words

Gas chromatography-mass spectrometry Honeys Carboxylic acids 5-Hydroxymethylfurfurol Proline 

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References

  1. [1]
    M. Morvai, I. Molnár-Perl, J. Chromatogr.520, 2011 (1990).CrossRefGoogle Scholar
  2. [2]
    I. Molnár-Perl, M. Morvai: Acta Alimentaria20, 62 (1991).Google Scholar
  3. [3]
    M. Morvai, I. Molnár-Perl, D. Knausz, J. Chromatogr.552, 337 (1991).CrossRefGoogle Scholar
  4. [4]
    M. Morvai, I. Molnár-Perl, Chromatographia34, 502 (1992).CrossRefGoogle Scholar
  5. [5]
    I. Molnár-Perl, M. Morvai: Food Addit. Contam.9, 505 (1992).Google Scholar
  6. [6]
    M. Morvai-Vitányi, I. Molnár-Perl, D. Knausz, P. Sass, Chromatographia37, 204 (1993).CrossRefGoogle Scholar
  7. [7]
    S. Tisza, I. Molnár-Perl, HRC17, 165 (1994).Google Scholar
  8. [8]
    S. Tisza, P. Sass, I. Molnár-Perl: J. Chromatogr.676, 461 (1994).CrossRefGoogle Scholar
  9. [9]
    S. Tisza, M. Friedman, P. Sass, I. Molnár-Perl, HRC19, 54 (1996).Google Scholar
  10. [10]
    I. Molnár-Perl, K. Horváth, Chromatographia45, 321 (1997).Google Scholar
  11. [11]
    K. Horváth, I. Molnár-Perl, Chromatographia45, 328 (1997).Google Scholar
  12. [12]
    I. Molnár-Perl, K. Horváth, R. Bartha, Chromatographia48, 101 (1998).Google Scholar
  13. [13]
    I. Molnár Perl, A. Vasanits, I. Molnár-Perl, Chromatographia48, 111 (1998).Google Scholar
  14. [14]
    M. L. Tourn, A. Lombard, F. Belliardo, M. Buffa, J. Apic. Res.19, 144 (1980).Google Scholar
  15. [15]
    K. Speer, A. Montag, Dtsch. Lebensm.-Rundsch.80, 103 (1984).Google Scholar
  16. [16]
    E. Steeg, A. Montag, Z. Lebensm. Unters. Forsch.184, 17 (1987).CrossRefGoogle Scholar
  17. [17]
    E. Steeg, A. Montag, Dtsch. Lebensm.-Rundsch.84, 103 (1988).Google Scholar
  18. [18]
    B. Talpay, Dtsch. Lebensm.-Rundsch.84, 41 (1988).Google Scholar
  19. [19]
    M. J. Amiot, S. Aubert, M. Gonnet, M. Tacchini, Apidologie20, 115 (1989).Google Scholar
  20. [20]
    E. Jörg, G. Sontag, Dtsch. Lebensm.-Rundsch.88, 179 (1992).Google Scholar
  21. [21]
    E. Jörg, G. Sontag, J. Chromatogr.635, 137 (1993).CrossRefGoogle Scholar
  22. [22]
    V. Petrov, Am. Bee J.112, 171 (1972).Google Scholar
  23. [23]
    J. Gilbert, M. J. Shepherd, M. A. Wallwork, R. G. Haris, J. Apic. Res.20, 125 (1981).Google Scholar
  24. [24]
    A. M. C. Davies, R. G. Harris, J. Apic. Res.21, 168 (1982).Google Scholar
  25. [25]
    A. Speer, A. Montag, Dtsch. Lebensm.-Rundsch.82, 248 (1986).Google Scholar
  26. [26]
    A. Pirini, L. S. Conte, O. Francioso, G. Lercker, HRC.15, 165 (1992).Google Scholar
  27. [27]
    M. Pawlowska, D. W. Armstrong, Chirality6, 270 (1994).CrossRefGoogle Scholar
  28. [28]
    M. Wootton, L. Ryall, J. Apic. Res.24, 120 (1985).Google Scholar
  29. [29]
    M. Lever, P. C. May, C. M. André, Anal. Biochem.144, 6 (1985).CrossRefGoogle Scholar
  30. [30]
    P. Vinas, N. Campillo, M. H. Córdoba, M. E. Candela, Food Chem.44, 67 (1992).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • K. Horváth
    • 1
  • I. Molnár-Perl
    • 1
  1. 1.Institute of Inorganic & Analytical ChemistryL. Eötvös UniversityBudapest 112Hungary

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