, Volume 40, Issue 1, pp 57–64 | Cite as

Molecular phylogeny of JapaneseAmanita species based on nucleotide sequences of the internal transcribed spacer region of nuclear ribosomal DNA

  • Takashi Oda
  • Chihiro Tanaka
  • Mitsuya Tsuda
Original Papers


The molecular phylogeny of 36 specimens of JapaneseAmanita species was studied based on nucleotide sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. The phylogenetic tree obtained supported the traditional classification systems of Bas (1969) and Singer (1986), which are based on morphological characters, in the division of the genusAmanita is divided into subgeneraAmanita andLepidella by the amyloidity of basidiospores. However, at section-level, we suggest that subgenusAmanita should be divided into three sections (Amanita, Vaginatae, andCaesareae). Our results also showed the necessity to modify the taxonomic treatments at section-level in the subgenusLepidella. It appears that the establishment ofA. muscaria andA. pantherina from a common ancestral species might be a very recent event, or these might be lower taxa of same species. As for three subspecies ofA. hemibapha and three varieties ofA. vaginata, it is necessary to grade up their taxonomical ranks from subspecies/variety to species. A new combination,A. javanica, is proposed forA. hemibapha subsp.javanica.

Key Words

Amanita Amanita javanica classification system ITS molecular phylogeny 


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Copyright information

© The Mycological Society of Japan 1999

Authors and Affiliations

  • Takashi Oda
    • 1
  • Chihiro Tanaka
    • 1
  • Mitsuya Tsuda
    • 1
  1. 1.Laboratory of Environmental Mycoscience, Faculty of AgricultureKyoto UniversityKyotoJapan

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